-
Journal of Bacteriology Oct 1967
Topics: Amino Acids; Carbohydrates; Cell Wall; Cellulose; Eukaryota; Glycosaminoglycans; Lipids; Microscopy, Phase-Contrast; Protein Hydrolysates; Proteins
PubMed: 4228053
DOI: 10.1128/jb.94.4.1272-1274.1967 -
The Biochemical Journal Mar 19731. A proteoglycan fraction (the proteoglycan subunit fraction) was prepared from extracts, with 0.15m-KCl (low-ionic-strength) and 0.5m-LaCl(3), 2.0m-CaCl(2) and... (Comparative Study)
Comparative Study
1. A proteoglycan fraction (the proteoglycan subunit fraction) was prepared from extracts, with 0.15m-KCl (low-ionic-strength) and 0.5m-LaCl(3), 2.0m-CaCl(2) and 4.0m-guanidinium chloride (high-ionic-strength), of bovine nasal cartilage by equilibrium-density-gradient centrifugation, essentially as described by Hascall & Sajdera (1969). 2. The use of different centrifugation times showed that near-equilibrium conditions were reached by 48h for the fractions prepared from the high-ionic-strength extracts. The fraction isolated from the low-ionic-strength extract required a longer centrifugation time to reach equilibrium conditions. 3. The composition of the proteoglycan fractions from the various extracts was compared by analyses of their carbohydrate and amino acid contents. Difference indices were calculated from the amino acid analysis to compare the degree of compositional relationship between the protein components of the proteoglycans. 4. Small compositional differences were found between the proteoglycans isolated from the various high-ionic-strength extracts. The protein content of the fractions from the CaCl(2) extract and the guanidinium chloride extract showed the greatest difference in this respect, although their amino acid analysis was similar. 5. The proteoglycan fraction isolated from the low-ionic-strength extract shows marked differences in composition from the fractions isolated from the high-ionic-strength extracts. Its protein and glucosamine contents were lower whereas its hexuronic acid and galactosamine contents were higher than those of the latter. It also exhibits major differences in its amino acid composition. The glucosamine:galactosamine ratio of the fraction from the low-ionic-strength extract indicates that it may be an almost exclusively chondroitin sulphate-proteoglycan. Its analysis correlates closely with that of a low-molecular-weight proteoglycan isolated from pig laryngeal cartilage by Tsiganos & Muir (1969). 6. The proteoglycan fractions from both the low- and high-ionic-strength extracts migrate as a single band in zone electrophoresis carried out in a sucrose-density gradient at both pH3.0 and pH7.0, although each showed evidence of band widening during the electrophoresis. All the proteoglycan fractions migrated with the same electrophoretic mobility at pH3.0, irrespective of the differences in composition between them. 7. The differences between the proteoglycans from the low- and high-ionic-strength extracts are discussed and the view is advanced that they may be due to association between predominantly chondroitin sulphate-proteoglycans and a keratan sulphate-enriched proteoglycan species.
Topics: Amino Acids; Animals; Calcium Chloride; Cartilage; Cattle; Centrifugation, Density Gradient; Chlorides; Chondroitin; Electrophoresis; Galactosamine; Glucosamine; Glycosaminoglycans; Guanidines; Hexosamines; Lanthanum; Nose; Osmolar Concentration; Potassium Chloride; Proteins; Solubility; Sulfuric Acids; Time Factors; Uronic Acids
PubMed: 4269049
DOI: 10.1042/bj1310541 -
Journal of Virology Dec 1971The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus. (Comparative Study)
Comparative Study
The nucleotide composition of the ribonucleic acid of three strains of rabies virus was determined and found to be similar to that of vesicular stomatitis virus.
Topics: Adenosine Monophosphate; Centrifugation, Density Gradient; Cytosine Nucleotides; Electrophoresis, Polyacrylamide Gel; Guanine Nucleotides; Nucleotides; Phosphorus Radioisotopes; RNA, Viral; Rabies virus; Uracil Nucleotides; Vesicular stomatitis Indiana virus
PubMed: 4366430
DOI: 10.1128/JVI.8.6.922-924.1971 -
The Journal of Biological Chemistry Apr 1969
Topics: Aging; Amino Acids; Animals; Carbohydrates; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Disaccharides; Electrophoresis; Fucose; Galactose; Glucose; Glycoproteins; Hexosamines; Kidney Glomerulus; Lens, Crystalline; Mannose; Membranes; Microbial Collagenase; Neuraminic Acids; Peptide Hydrolases; Peptides; Polysaccharides
PubMed: 4305665
DOI: No ID Found -
The Journal of Biological Chemistry Jan 1973
Topics: Amino Acids; Arginine; Aspartic Acid; Carbohydrates; Chromatography, Gel; Electrophoresis, Polyacrylamide Gel; Fruit; Glutamates; Hydrogen-Ion Concentration; Isoelectric Focusing; Lysine; Molecular Weight; Peptides; Plant Proteins; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Taste
PubMed: 4684691
DOI: No ID Found -
Journal of Biochemistry Jun 1974
Topics: Chromatography, Gas; Chromatography, Thin Layer; Cryptococcus; Cryptococcus neoformans; Fatty Acids; Glycerides; Magnetic Resonance Spectroscopy; Optical Rotation; Phosphatidic Acids; Phospholipids; Phosphoric Acids; Phosphorus; Photometry; Spectrophotometry, Infrared
PubMed: 4372236
DOI: 10.1093/oxfordjournals.jbchem.a130513 -
The Journal of Biological Chemistry Nov 1969
Topics: Amino Alcohols; Amino Sugars; Blood Group Antigens; Boron Compounds; Chemical Phenomena; Chemistry; Chromatography, Gel; Erythrocytes; Galactose; Glycoproteins; Hexosamines; Humans; Hydrogen-Ion Concentration; Methods; Models, Structural; Molecular Weight; Neuraminic Acids; Oligosaccharides; Peptides
PubMed: 5350948
DOI: No ID Found -
The Journal of Biological Chemistry Feb 1973
Topics: Animals; Cattle; Cell Nucleus; Chromatin; Circular Dichroism; DNA; Micrococcal Nuclease; Nucleic Acid Conformation; Sodium Chloride; Solubility; Spectrophotometry, Ultraviolet; Spectrum Analysis; Thymus Gland; Urea
PubMed: 4684705
DOI: No ID Found -
The Biochemical Journal Sep 1976Proteoglycan was prepared from three pools of normal human intervertebral discs by extraction with buffered 4M-guanidinium chloride followed by CsCl-density-gradient...
Proteoglycan was prepared from three pools of normal human intervertebral discs by extraction with buffered 4M-guanidinium chloride followed by CsCl-density-gradient ultracentrifugation. Chromatography on agarose (Bio-Gel A-150m) and on DEAE-cellulose suggested a single polydisperse proteoglycan species. The intrinsic viscosities of three preparations were 166, 122 and 168 ml/g. After degradation with 0.5M-KOH containing 0.02M-NaBH4, the glycosaminoglycans were recovered quantitatively and their Ca2+ salts separated into a hexuronate-rich fraction (fraction 1), which was precipitated in 0-45% (v/v) ethanol, and a hexose-rich fraction (fraction2), which was precipitated in 45-70% (v/v) ethanol. Qualitative and quantitative analyses of the glycosaminoglycans revealed fraction 1 to be chondroitin sulphate, and fraction 2 to be keratan sulphate; the latter was contaminated with protein and possibly a small amount of another glycosaminoglycan. For both glycosaminoglycans, plots of log(mol.wt.) against weight fell close to a normal distribution. The mode for chondroitin sulphate was close to 20000; that for keratan sulphate, 10000. A threefold range of molecular weight included the central 16-84% [+/- 1 S.D. of log(mol.wt.)] of the weight of both fractions.
Topics: Adult; Chondroitin Sulfates; Glycosaminoglycans; Hexosamines; Hexoses; Hexuronic Acids; Humans; Intervertebral Disc; Keratan Sulfate; Male; Middle Aged; Molecular Weight; Nitrogen; Proteoglycans; Viscosity
PubMed: 985415
DOI: 10.1042/bj1570753 -
Microbiology and Immunology 1983
Comparative Study
Topics: Amino Acid Sequence; Amino Acids; Carbohydrates; Chemical Phenomena; Chemistry; Peptidoglycan; Propionibacterium; Species Specificity
PubMed: 6633302
DOI: 10.1111/j.1348-0421.1983.tb00625.x