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Frontiers in Endocrinology 2022This study aimed at investigating the association between testosterone levels and gut microbiota in male patients with type 2 diabetes mellitus (T2DM) and providing a... (Observational Study)
Observational Study
OBJECTIVE
This study aimed at investigating the association between testosterone levels and gut microbiota in male patients with type 2 diabetes mellitus (T2DM) and providing a new strategy to elucidate the pathological mechanism of testosterone deficiency in T2DM patients.
METHODS
In an observational study including 46 T2DM male patients, the peripheral venous blood and fecal samples of all subjects were collected. The V3-V4 regions of bacterial 16S rDNA were amplified and sequenced. Alpha and beta diversities were calculated by QIIME software. The possible association between gut microbial community and clinical indicators was assessed using the Spearman correlation coefficient. The association between the relative abundance of bacteria and testosterone levels was discovered using linear regression analysis in R language.
RESULTS
There was no substantial difference in alpha and beta diversity. and were significantly much higher in the testosterone deficiency group. Linear regression analysis showed that the abundance of at the phylum level and at the order level were negatively correlated with testosterone level. After correcting for C-reactive protein (CRP) and homeostatic model assessment of insulin resistance (HOMA-IR), the relative abundance of still had a significant negative correlation with testosterone level. Meanwhile, at the genus level, , , and had a statistically significant negative association with testosterone level, respectively. was positively associated with FPG and total cholesterol level. was found positively associated with insulin, connecting peptide, and index of homeostatic model assessment of insulin resistance.
CONCLUSION
T2DM patients with testosterone deficiency have different gut microbiota compositions compared with T2DM patients alone. Low serum testosterone patients tend to have an increased abundance of opportunistic pathogens, which may be related to the occurrence and development of testosterone deficiency.
Topics: Diabetes Mellitus, Type 2; Gastrointestinal Microbiome; Humans; Insulin Resistance; Male; Microbiota; Testosterone
PubMed: 35399957
DOI: 10.3389/fendo.2022.836485 -
BMC Microbiology Feb 2020Bergeyella cardium infection is becoming increasingly prevalent in patients with infective endocarditis, suggesting its significance in disease pathogenesis. However,...
BACKGROUND
Bergeyella cardium infection is becoming increasingly prevalent in patients with infective endocarditis, suggesting its significance in disease pathogenesis. However, few studies have fully characterized this species.
RESULTS
Herein, we report the morphological and physiological characteristics, as well as whole genome sequencing of a newly identified B. cardium HPQL strain isolated from a patient with infective endocarditis. Results from the cellular morphology and biochemical analysis provide basic knowledge on the new pathogen. The whole genome sequencing of B. cardium HPQL consists of a circular chromosome with a total length of 2,036,890 bp. No plasmid was detected. Comparative genomics were carried out then. Antibiotics resistance related genes, pathogenesis related genes, predicted insertion sequences, genome islands and predicted CRISPRs sequences were demonstrated. To our knowledge, this is the first study to provide a complete genome sequence for Bergeyella spp.
CONCLUSIONS
This study provides fundamental phenotypic and genomic information for the newly identified fastidious infective endocarditis causative bacteria, B. cardium. Our results provide insights into effective clinical diagnosis and treatment of this pathogen.
Topics: Drug Resistance, Bacterial; Endocarditis; Flavobacteriaceae; Genome Size; Genome, Bacterial; Humans; Male; Middle Aged; Phenotype; Phylogeny; Virulence; Whole Genome Sequencing
PubMed: 32050896
DOI: 10.1186/s12866-020-1715-0 -
Frontiers in Microbiology 2021Gliomas are the most prevalent form of primary malignant brain tumor, which currently have no effective treatments. Evidence from human studies has indicated that oral...
Gliomas are the most prevalent form of primary malignant brain tumor, which currently have no effective treatments. Evidence from human studies has indicated that oral microbiota is closely related to cancers; however, whether oral microbiota plays a role in glioma malignancy remains unclear. The present study aimed to investigate the association between oral microbiota and grade of glioma and examine the relationship between malignancy-related oral microbial features and the isocitrate dehydrogenase 1 (IDH1) mutation in glioma. High-grade glioma (HGG; =23) patients, low-grade glioma (LGG; =12) patients, and healthy control (HCs; =24) participants were recruited for this case-control study. Saliva samples were collected and analyzed for 16S ribosomal RNA (rRNA) sequencing. We found that the shift in oral microbiota β-diversity was associated with high-grade glioma (=0.01). The phylum Patescibacteria was inversely associated with glioma grade (LGG and HC: =0.035; HGG and HC: <0.01). The genera (LGG and HC: =0.043; HGG and HC: <0.01) and (LGG and HC: =0.044; HGG and HC: <0.01) were inversely associated with glioma grades. The genera and were significantly more positively correlated with the IDH1 mutation in gliomas when compared with the IDH1-wild-type group. We further identified five oral microbial features (, , , and ) that accurately discriminated HGG from LGG (area under the curve [AUC]: 0.63, 95% confidence interval [CI]: 0.44-0.83) and HCs (AUC: 0.79, 95% CI: 0.68-0.92). The functional prediction analysis of oral bacterial communities showed that genes involved in cell adhesion molecules (<0.001), extracellular matrix molecule-receptor interaction (<0.001), focal adhesion (<0.001), and regulation of actin cytoskeleton (<0.001) were associated with glioma grades, and some microbial gene functions involving lipid metabolism and the adenosine 5'-monophosphate-activated protein kinase signaling pathway were significantly more enriched in IDH1 mutant gliomas than compared with the IDH1-wild-type gliomas. In conclusion, our work revealed oral microbiota features and gene functions that were associated with glioma malignancy and the IDH1 mutation in glioma.
PubMed: 34733261
DOI: 10.3389/fmicb.2021.746568 -
Open Forum Infectious Diseases Apr 2019is a new species in the family Flavobacteriaceae that was recently described in 3 cases of native valve infective endocarditis. We report the first case of prosthetic...
is a new species in the family Flavobacteriaceae that was recently described in 3 cases of native valve infective endocarditis. We report the first case of prosthetic valve endocarditis, provide the first draft genome of this species, and review the microbiologic characteristics of this emerging pathogen.
PubMed: 31024974
DOI: 10.1093/ofid/ofz134 -
BMC Infectious Diseases Apr 2017Bergeyella zoohelcum is an aerobic, Gram-negative bacterium that is frequently isolated from the upper respiratory tract of dogs, cats and other mammals. Clinically, B.... (Review)
Review
BACKGROUND
Bergeyella zoohelcum is an aerobic, Gram-negative bacterium that is frequently isolated from the upper respiratory tract of dogs, cats and other mammals. Clinically, B. zoohelcum has been reported causing cellulitis, tenosynovitis, leg abscess and septicemia, which is closely connected with animal bites. Here we describe a case of bacteremia in an infective endocarditis (IE) patient caused by B. zoohelcum, in China.
CASE PRESENTATION
A 27-year-old infective endocarditis woman who had no history of dog bite nor other mammal exposure suffered bacteremia caused by B. zoohelcum. This patient, without evidence of polymicrobial infection, was treated with cefuroxime and had a good outcome.
CONCLUSIONS
B. zoolhelcum bacteremia is rarely reported in IE patients. Our report expands the range of known bacterial causes of infective endocarditis.
Topics: Adult; Animals; Bacteremia; Bites and Stings; Cats; Cellulitis; China; Dogs; Endocarditis, Bacterial; Female; Gram-Negative Bacteria; Humans; Male; Middle Aged
PubMed: 28403835
DOI: 10.1186/s12879-017-2391-z -
Journal of Oral Microbiology 2022Burning mouth syndrome (BMS) is a chronic pain condition accompanied by unpleasant burning sensations of the oral mucosa. While multiple factors were proposed for the...
Burning mouth syndrome (BMS) is a chronic pain condition accompanied by unpleasant burning sensations of the oral mucosa. While multiple factors were proposed for the etiology, evidence suggested a neuropathic pain origin while others suspected the use of antibiotics as the underlying cause. Interestingly, several reports demonstrated the intimate interaction of the nervous system and the microbiome. The current study aims to elucidate the correlation of the oral microbiome with the pathophysiology of the primary BMS. Microbiome samples obtained from the unstimulated whole saliva of 19 primary BMS patients and 22 healthy controls were sequenced and analyzed of the V3-V4 region of 16S rRNA gene. There was a distinct difference in the microbial composition between the BMS and the control groups at all taxonomic levels. Alpha diversity indexes of the oral microbiome were significantly lower in the BMS group. The samples were readily distinguished by multidimensional scaling analysis and linear discriminant analysis effect size. , , , and genus were dominant in the BMS group, while , , , , and genus were more abundant in the healthy group. Distinct microbiome signatures of BMS patients suggested a diagnostic value and a potential role in the pathogenesis of BMS.
PubMed: 35341209
DOI: 10.1080/20002297.2022.2052632 -
Journal of Applied Microbiology Mar 1998Eighty-five catalase- and oxidase-positive Gram-negative rods and cocci susceptible to penicillin G were isolated from a variety of food sources. The phenotypic... (Comparative Study)
Comparative Study
Eighty-five catalase- and oxidase-positive Gram-negative rods and cocci susceptible to penicillin G were isolated from a variety of food sources. The phenotypic relationships of these isolates with reference cultures of Bergeyella-like, Chryseobacterium, Empedobacter, Myroides, Moraxella, Sphingobacterium and Weeksella-like strains were examined by numerical taxonomy. Seventy-three isolates were recovered in five groups; 80% of the isolates clustered in groups 1, 2 and 3 and produced indole, bearing a strong resemblance to Weeksella and Bergeyella. They could not, however, be regarded as belonging to the known species of W. virosa and B. zoohelcum. It is suggested that three species may be necessary to accommodate the environmental Weeksella- or Bergeyella-like bacteria. The isolates in groups 4 and 5 had white colonies and were unable to produce indole, in this way resembling the Moraxella genus.
Topics: Bacterial Typing Techniques; Food Microbiology; Gram-Negative Aerobic Rods and Cocci; Gram-Negative Bacteria; Moraxella
PubMed: 9721639
DOI: 10.1046/j.1365-2672.1998.00346.x -
Case Reports in Ophthalmological... 2023We report a case of bacterial keratitis secondary to an undescribed sp. spp. are not easily cultured, and many reports have identified unculturable isolates through...
We report a case of bacterial keratitis secondary to an undescribed sp. spp. are not easily cultured, and many reports have identified unculturable isolates through broad-range bacterial polymerase chain reaction (PCR). . A healthy 29-year-old male was attempting to repair an acrylic cannabis water pipe when it shattered and a fragment hit him in the left eye. Two weeks later, he presented with foreign body sensation, scleral injection, and photophobia that were refractory to prolonged corticosteroid therapy. Following a subconjunctival triamcinolone injection, the patient developed a hypopyon and multifocal, midstromal, epithelized corneal infiltrates. Broad-range PCR of the aqueous fluid detected deoxyribonucleic acid closely matching the genus. Empiric treatment directed toward gram-negative bacteria led to the clinical resolution of the inflammation. This is the first reported case of ocular inflammation secondary to a spp.. As broad-range PCR testing becomes more accessible, we anticipate that additional PCR-positive and culture-negative scenarios will occur.
PubMed: 37273837
DOI: 10.1155/2023/3288984 -
Animal Microbiome May 2021Oral diseases are common in dogs, with microbiota playing a prominent role in the disease process. Oral cavity habitats harbor unique microbiota populations that have...
BACKGROUND
Oral diseases are common in dogs, with microbiota playing a prominent role in the disease process. Oral cavity habitats harbor unique microbiota populations that have relevance to health and disease. Despite their importance, the canine oral cavity microbial habitats have been poorly studied. The objectives of this study were to (1) characterize the oral microbiota of different habitats of dogs and (2) correlate oral health scores with bacterial taxa and identify what sites may be good options for understanding the role of microbiota in oral diseases. We used next-generation sequencing to characterize the salivary (SAL), subgingival (SUB), and supragingival (SUP) microbial habitats of 26 healthy adult female Beagle dogs (4.0 ± 1.2 year old) and identify taxa associated with periodontal disease indices.
RESULTS
Bacterial species richness was highest for SAL, moderate for SUB, and lowest for SUP samples (p < 0.001). Unweighted and weighted principal coordinates plots showed clustering by habitat, with SAL and SUP samples being the most different from one another. Bacteroidetes, Proteobacteria, Firmicutes, Fusobacteria, Actinobacteria, and Spirochaetes were the predominant phyla in all habitats. Paludibacter, Filifactor, Peptostreptococcus, Fusibacter, Anaerovorax, Fusobacterium, Leptotrichia, Desulfomicrobium, and TG5 were enriched in SUB samples, while Actinomyces, Corynebacterium, Leucobacter, Euzebya, Capnocytophaga, Bergeyella, Lautropia, Lampropedia, Desulfobulbus, Enhydrobacter, and Moraxella were enriched in SUP samples. Prevotella, SHD-231, Helcococcus, Treponema, and Acholeplasma were enriched in SAL samples. p-75-a5, Arcobacter, and Pasteurella were diminished in SUB samples. Porphyromonas, Peptococcus, Parvimonas, and Campylobacter were diminished in SUP samples, while Tannerella, Proteocalla, Schwartzia, and Neisseria were diminished in SAL samples. Actinomyces, Corynebacterium, Capnocytophaga, Leptotrichia, and Neisseria were associated with higher oral health scores (worsened health) in plaque samples.
CONCLUSIONS
Our results demonstrate the differences that exist among canine salivary, subgingival plaque and supragingival plaque habitats. Salivary samples do not require sedation and are easy to collect, but do not accurately represent the plaque populations that are most important to oral disease. Plaque Actinomyces, Corynebacterium, Capnocytophaga, Leptotrichia, and Neisseria were associated with higher (worse) oral health scores. Future studies analyzing samples from progressive disease stages are needed to validate these results and understand the role of bacteria in periodontal disease development.
PubMed: 34001282
DOI: 10.1186/s42523-021-00100-9 -
Cancer Epidemiology, Biomarkers &... Jul 2018The oral microbiota play a central role in oral health, and possibly in carcinogenesis. Research suggests that coffee and tea consumption may have beneficial health...
The oral microbiota play a central role in oral health, and possibly in carcinogenesis. Research suggests that coffee and tea consumption may have beneficial health effects. We examined the associations of these common beverages with the oral ecosystem in a large cross-sectional study. We assessed oral microbiota in mouthwash samples from 938 participants in two U.S. cohorts using 16S rRNA gene sequencing. Coffee and tea intake were assessed from food frequency questionnaires. We examined associations of coffee and tea intake with overall oral microbiota diversity and composition using linear regression and permutational MANOVA, respectively, and with taxon abundance using negative binomial generalized linear models; all models adjusted for age, sex, cohort, body mass index, smoking, ethanol intake, and energy intake. Higher tea intake was associated with greater oral microbiota richness ( = 0.05) and diversity ( = 0.006), and shifts in overall community composition ( = 0.002); coffee was not associated with these microbiome parameters. Tea intake was associated with altered abundance of several oral taxa; these included Fusobacteriales, Clostridiales, and (higher with increasing tea) and Bifidobacteriaceae, , Lactobacillales, and (lower with increasing tea). Higher coffee intake was only associated with greater abundance of and Synergistetes. In the largest study to date of tea and coffee consumption in relation to the oral microbiota, the microbiota of tea drinkers differed in several ways from nondrinkers. Tea-driven changes to the oral microbiome may contribute to previously observed associations between tea and oral and systemic diseases, including cancers. .
Topics: Aged; Coffee; Cross-Sectional Studies; Female; Humans; Male; Middle Aged; Risk Factors; Surveys and Questionnaires; Tea
PubMed: 29703763
DOI: 10.1158/1055-9965.EPI-18-0184