-
Insects May 2022Grasshoppers (Insecta, Orthoptera, Acridoidea) are a large group of agricultural and animal husbandry pests. They have a large food intake with high utilization of...
Grasshoppers (Insecta, Orthoptera, Acridoidea) are a large group of agricultural and animal husbandry pests. They have a large food intake with high utilization of plants fibers. However, the composition of the grasshopper gut microbial community, especially the relationship between gut microbial community and cellulose digestibility, remains unclear. In this research, 16S rRNA gene sequences were used to determine the intestinal microbial diversity of , , and , and Spearman correlation analysis was performed between the intestinal microbes of grasshoppers and the digestibility of cellulose and hemicellulose. The results showed that Proteobacteria was the dominant phylum and was the dominant genus in the guts of the four species of grasshoppers; there was no significant difference in the species composition of the gut microbes of the four species of grasshoppers. Spearman correlation analysis showed that and were significantly correlated with cellulose digestibility. , , and were significantly associated with hemicellulose digestibility. Our results confirmed that the gut microbes of grasshoppers were correlated with the digestibility of cellulose and hemicellulose, and indicated that grasshoppers may have the potential to develop into bioreactors, which can be applied to improve straw utilization efficiency in the future.
PubMed: 35621768
DOI: 10.3390/insects13050432 -
Cureus Jun 2021is a large genus that is not often involved in pathogenesis, however, since 1991 there have been several case reports of -associated illness, most often due to...
is a large genus that is not often involved in pathogenesis, however, since 1991 there have been several case reports of -associated illness, most often due to bacteremia in the setting of an immunocompromised patient with a central venous catheter (CVC). Here we detail the case of an elderly woman with many comorbidities and a peripherally inserted central catheter (PICC) line for over four years, who presented with septic shock and bacteremia. In nearly all previous cases of bacteremia it was thought to be due to a CVC which was removed as part of the treatment in conjunction with antibiotics. In this case, the patient was treated with empiric antibiotics and her blood cultures cleared within 48 hours without catheter removal or antibiotic-lock therapy. The clinical outcome was favorable at 50 days follow-up.
PubMed: 34336495
DOI: 10.7759/cureus.16004 -
Journal of Dairy Science Jun 2018A commercial blue-veined cheese made from unpasteurized milk was examined by conventional culturing and PCR denaturing gradient gel electrophoresis analysis of the...
A commercial blue-veined cheese made from unpasteurized milk was examined by conventional culturing and PCR denaturing gradient gel electrophoresis analysis of the bacterial community 16S rRNA genes using 3 primer sets, V3, V4V5, and V6V8. Genomic DNA for amplification was extracted directly from raw milk, starter culture, cheese at different stages of production, fully ripened cheese, and from the cultured cells grown on various media. The outer rind was sampled separately from the inner white core and blue veins. A diverse microbiota containing Lactococcus lactis ssp. lactis, Lactobacillus plantarum, Lactobacillus curvatus, Staphylococcus gallinarum, Staphylococcus devriesei, Microbacterium sp., Sphingobacterium sp., Mycetocola sp., Brevundimonas sp., Enterococcus faecalis, Proteus sp., and Kocuria sp. was detected in the raw milk using culturing methods, but only Lactococcus lactis ssp. lactis, Lactobacillus plantarum, and Enterococcus faecalis survived to the final cheese and were detected both in the core and the rind. Using PCR denaturing gradient gel electrophoresis analysis of the cheese process samples, Staphylococcus equorum and Enterococcus durans were found in the rind of prepiercing samples but not in the core and veins; after piercing, these species were found in all parts of the cheese but survived only in the rind when the cheese was fully ripened. Brevibacterium sp., Halomonas sp., Acinetobacter sp., Alkalibacterium sp., and Corynebacterium casei were identified only by PCR denaturing gradient gel electrophoresis and not cultured from the samples. Brevibacterium sp. was initially identified in the cheese postpiercing (core and veins), Halomonas sp. was found in the matured cheese (rind), and Acinetobacter sp., Alkalibacterium sp., and Corynebacterium casei were also found in the prepiercing samples (rind) and then found through the subsequent process stages. The work suggests that in this raw milk cheese, a limited community from the milk survive to the final cheese, with salt addition and handling contributing to the final cheese consortium.
Topics: Animals; Bacteria; Biodiversity; Cheese; Colony Count, Microbial; DNA, Bacterial; Denaturing Gradient Gel Electrophoresis; Food Microbiology; Microbiota; Milk; Polymerase Chain Reaction; RNA, Ribosomal, 16S; United Kingdom
PubMed: 29550118
DOI: 10.3168/jds.2017-14104 -
3 Biotech Jan 2020Microbial strains capable of degrading petroleum hydrocarbons were isolated from the Yellow River Delta and screened for bio-surfactant production. The...
Microbial strains capable of degrading petroleum hydrocarbons were isolated from the Yellow River Delta and screened for bio-surfactant production. The bio-surfactant-producing characteristics of the isolates were evaluated, and all the isolates which could produce bio-surfactant were identified by 16S rRNA gene sequencing. The results showed that the isolates belong to sp. (72%), sp. (0.16%), sp. (0.06%) and sp. (0.06%). The biodegradability of crude oil, gasoline, diesel oil and other hydrocarbons by microbial strains were studied, among which the biodegrading ability of strain P1 and strain P19 is higher than other strains. Both strains P1 and P19 can degrade -hexane and -hexadecane effectively and have wide substrate extensiveness. In addition, Ni promoted the biodegradability of toluene by both strain P1 and strain P19, while Pb inhibited the growth of strain P19 and decreased its ability to biodegrade toluene. The studies revealed that microbes including strain P1 and strain P19 can be utilized in bioremediation of co-contaminated water with petroleum and heavy metals including Ni and Pb.
PubMed: 31879582
DOI: 10.1007/s13205-019-2011-2 -
Microbiology Resource Announcements May 2022We report the complete and circularized genome sequences of Brevibacterium frigoritolerans Ant232, generated using a combination of Illumina and PacBio platforms. The...
We report the complete and circularized genome sequences of Brevibacterium frigoritolerans Ant232, generated using a combination of Illumina and PacBio platforms. The high-quality complete genome consists of a circular 5,586,945-bp chromosome and a 305,498-bp plasmid, with G+C contents of 40.66% and 36.8%, respectively.
PubMed: 35389269
DOI: 10.1128/mra.00152-22 -
Research in Pharmaceutical Sciences Aug 2017The marine environment covers three quarters of the surface of the planet and is estimated to be home to more than 80% of life but yet it remains largely unexplored. It...
The marine environment covers three quarters of the surface of the planet and is estimated to be home to more than 80% of life but yet it remains largely unexplored. It harbours a number of macro and microorganisms that have developed unique metabolic abilities to ensure their survival in diverse and hostile habitats, resulting in the biosynthesis of an array of secondary metabolites with specific activities. In this study, pigment forming bacterial strains were isolated from the sea surface inter tidal zones at different sampling sites along the Visakhapatnam coastal region. The bacterial isolates showed various types of colour pigments like pink, yellow, orange, and brown. Out of 26 pigmented isolates obtained, the bacterial isolate with bright yellow pigmentation was selected for further study. This strain was identified as by using morphological, physiological, biochemical and 16s rRNA sequencing methods. The pigment was extracted in methanol solvent and antibacterial activity of the pigments extracted from the bacteria was determined and it was found active against pathogenic bacteria. The pigment extract was tested for anti-inflammatory activity and was effective.
PubMed: 28855939
DOI: 10.4103/1735-5362.212045 -
Journal of Biochemistry Feb 1962
Topics: Amino Acids; Bacteria; Brevibacterium; Brevibacterium flavum; Cytoplasm; Glutamates
PubMed: 13911886
DOI: 10.1093/oxfordjournals.jbchem.a127506 -
Environment International Nov 2023Atrazine residues can pose serious threats to soil ecology and human health. Currently, the underlying relationship between soil microbial communities and the...
Atrazine residues can pose serious threats to soil ecology and human health. Currently, the underlying relationship between soil microbial communities and the degradation genes associated with atrazine degradation remains unclear. In this study, the degradation characteristics of atrazine was investigated in ten different soil types. Further, diversity and abundance of degradation genes and succession of the bacterial community were also studied. The degradation of 10 mg/kg atrazine in different soil types exhibited an initial rapid trend followed by a gradual slowdown, adhering to the first-order kinetic equation. Atrazine significantly increased the absolute abundance of atz degradation genes. The increase in the absolute abundance of atzC gene was the largest, whereas that of atzA gene was the smallest, and the trzD gene was only detected in the Binzhou loam soil. Co-occurrence network analysis showed that the number of potential bacterial hosts of atzC was the highest compared with the other atz genes. Atrazine also altered the structural composition of the soil microbial community. The relative abundances of Ochrobactrum, Nocardiopsis, Lactobacillus, and Brevibacterium was increased in the atrazine-treated soils, while those of Conexibate, Solirubacter, and Micromonospora was decreased significantly compared with the control. Additionally, four atrazine-degrading bacterial strains Rhizobium AT1, Stenotrophomonas AT2, Brevibacterium AT3, and Bacillus AT4 were isolated from the atrazine-treated soils. After 14 d for inoculation, their degradation rate for 10 mg/L atrazine ranged from 17.56 % to 30.55 %. Moreover, the relative abundances of the bacterial genera, including these four isolates, in the atrazine-treated soil were significantly higher than those in the control, indicating that they were involved in the synergistic degradation of atrazine in the soil. This study revealed the degradation characteristics of atrazine, distribution of degradation genes, and succession of microbial communities, and explored the internal relationship between microbial community structure and atrazine degradation mechanisms in different soil types.
Topics: Humans; Atrazine; Herbicides; Soil; Soil Microbiology; Bacteria; Biodegradation, Environmental; Soil Pollutants
PubMed: 37948867
DOI: 10.1016/j.envint.2023.108303 -
Journal of Dairy Science Aug 2012To evaluate the effects of temperature and relative humidity (RH) on microbial and biochemical ripening kinetics, Camembert-type cheeses were prepared from pasteurized...
To evaluate the effects of temperature and relative humidity (RH) on microbial and biochemical ripening kinetics, Camembert-type cheeses were prepared from pasteurized milk seeded with Kluyveromyces marxianus, Geotrichum candidum, Penicillium camemberti, and Brevibacterium aurantiacum. Microorganism growth and biochemical changes were studied under different ripening temperatures (8, 12, and 16°C) and RH (88, 92, and 98%). The central point runs (12°C, 92% RH) were both reproducible and repeatable, and for each microbial and biochemical parameter, 2 kinetic descriptors were defined. Temperature had significant effects on the growth of both K. marxianus and G. candidum, whereas RH did not affect it. Regardless of the temperature, at 98% RH the specific growth rate of P. camemberti spores was significantly higher [between 2 (8°C) and 106 times (16°C) higher]. However, at 16°C, the appearance of the rind was no longer suitable because mycelia were damaged. Brevibacterium aurantiacum growth depended on both temperature and RH. At 8°C under 88% RH, its growth was restricted (1.3 × 10(7) cfu/g), whereas at 16°C and 98% RH, its growth was favored, reaching 7.9 × 10(9) cfu/g, but the rind had a dark brown color after d 20. Temperature had a significant effect on carbon substrate consumption rates in the core as well as in the rind. In the rind, when temperature was 16°C rather than 8°C, the lactate consumption rate was approximately 2.9 times higher under 88% RH. Whatever the RH, temperature significantly affected the increase in rind pH (from 4.6 to 7.7 ± 0.2). At 8°C, an increase in rind pH was observed between d 6 and 9, whereas at 16°C, it was between d 2 and 3. Temperature and RH affected the increasing rate of the underrind thickness: at 16°C, half of the cheese thickness appeared ripened on d 14 (wrapping day). However, at 98% RH, the underrind was runny. In conclusion, some descriptors, such as yeast growth and the pH in the rind, depended solely on temperature. However, our findings highlight the fact that the interactions between temperature and RH played a role in P. camemberti sporulation, B. aurantiacum growth, carbon substrate consumption rates, and the thickening of the cheese underrind. Moreover, the best ripening conditions to achieve an optimum between microorganism growth and biochemical kinetics were 13°C and 94% RH.
Topics: Brevibacterium; Cheese; Colony Count, Microbial; Food Microbiology; Geotrichum; Humidity; Hydrogen-Ion Concentration; Kluyveromyces; Lactic Acid; Lactose; Penicillium; Pilot Projects; Statistics, Nonparametric; Temperature
PubMed: 22818481
DOI: 10.3168/jds.2012-5368 -
Current Research in Microbial Sciences 2022This study reports the diversity of cultivable endophytic bacteria associated with yellow iris ( L.) by using 16S rRNA gene analysis and their plant beneficial traits....
This study reports the diversity of cultivable endophytic bacteria associated with yellow iris ( L.) by using 16S rRNA gene analysis and their plant beneficial traits. The 16S rRNA sequence similarities of endophytic bacteria isolated from the leaves and roots of yellow iris showed that the isolates belonged to the genera and . The endophytic bacteria HRT18, HRT8, HRT13, and HST13 exhibited antimicrobial activity against five plant pathogenic fungi and . They also demonstrated the capability to produce chitinase, protease, glucanase, lipase, HCN, and indole-3-acetic acid (IAA). Thirteen isolates (46%) produced IAA, and the most active IAA producers were , and . The IAA producing bacterial isolates stimulated root and shoot growth of garden cress. Our findings suggest that medicinal plants could be a promising source for isolating plant-beneficial bacteria that can be used to enhance the growth and protect plants against soil-borne pathogens.
PubMed: 35909614
DOI: 10.1016/j.crmicr.2022.100133