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Brain Sciences Feb 2020The maximum eigenvalue of the adjacency matrix (AM) has been supposed to contain rich information about the corresponding network. An experimental study focused on...
The maximum eigenvalue of the adjacency matrix (AM) has been supposed to contain rich information about the corresponding network. An experimental study focused on revealing the meaning and application of the maximum eigenvalue is missing. To this end, AM was constructed using mutual information (MI) to determine the functional connectivity with electroencephalogram (EEG) data recorded with a mental fatigue model, and then was converted into both binary and weighted brain functional network (BFN) and corresponding random networks (RNs). Both maximum eigenvalue and corresponding network characters in BFNs and RNs were considered to explore the changes during the formation of mental fatigue. The results indicated that large maximum eigenvalue means more edges in the corresponding network, along with a high degree and a short characteristic path length both in weighted and binary BFNs. Interestingly, the maximum eigenvalue of AM was always a little larger than that of the corresponding random matrix (RM), and had an obvious linearity with the sum of the AM elements, indicating that the maximum eigenvalue can be able to distinguish the network structures which have the same mean degree. What is more, the maximum eigenvalue, which increased with the deepening of mental fatigue, can become a good indicator for mental fatigue estimation.
PubMed: 32050462
DOI: 10.3390/brainsci10020092 -
Orphanet Journal of Rare Diseases Sep 2022Major challenges to health care access include low health insurance literacy, prohibitive costs, and insurance barriers. Niemann-Pick disease (NPD), comprising acid...
BACKGROUND
Major challenges to health care access include low health insurance literacy, prohibitive costs, and insurance barriers. Niemann-Pick disease (NPD), comprising acid sphingomyelinase deficiency (ASMD) and Niemann-Pick type C (NPC), is a group of rare, autosomal recessive, highly heterogeneous, neurovisceral, life-threatening, relentlessly progressive lysosomal disorders. Patients experience debilitating systemic and neurological symptoms and substantial emotional and financial stress. Currently, these multifaceted disorders are managed symptomatically as there are no approved therapies. Given the considerable disease burden of NPD, timely access to quality health care is paramount for improving outcomes in these life-threatening disorders. Understanding health insurance literacy and access challenges among patients with NPD and their caregivers is a first step to overcoming treatment barriers.
RESULTS
Patients from the Niemann-Pick community participated in a health insurance literacy survey and follow-up telephone interviews on perceived access challenges. Of the 79 respondents who completed the survey, 67 participated in interviews. All respondents had stable health insurance coverage. However, 61% of respondents were unaware of Medicaid waivers and did not avail of them. Overall, 50% of respondents with childhood onset NPC selected Medicaid/Medicare and private insurance; 35% utilized Medicaid waivers. Most respondents with ASMD had private insurance only. Although the Niemann-Pick community demonstrated greater health insurance literacy than the general population, knowledge gaps exist in calculating insurance coverage, out-of-pocket maximums, and defining a formulary. The most frequently cited access burden was the process of obtaining medical care and services. Among respondents with ASMD, the greatest access burden was fear of unavailability of or access to medications and treatment. Access challenges adversely impacted patients' mental health and exacerbated physical symptoms. Delays and denials in obtaining essential medication, equipment, and services contributed to disease progression. Caregivers faced burnout and often questioned the utility of their advocacy.
CONCLUSIONS
This study identified knowledge gaps in health insurance literacy and challenges to access medication and health care services among individuals impacted by NPD. Patients and caregivers need the knowledge and skills to navigate a complicated health care system, understand their rights to medication and services and, ultimately, benefit from improved outcomes, especially in a post-drug approval era.
Topics: Aged; Caregivers; Child; Health Services; Humans; Insurance, Health; Medicare; Niemann-Pick Disease, Type A; Niemann-Pick Diseases; United States
PubMed: 36056366
DOI: 10.1186/s13023-022-02490-8 -
International Journal of Environmental... Apr 2022Based on comparisons to moderate continuous exercise (MICT), high-intensity interval training (HIIT) is becoming a worldwide trend in physical exercise. This raises... (Review)
Review
Based on comparisons to moderate continuous exercise (MICT), high-intensity interval training (HIIT) is becoming a worldwide trend in physical exercise. This raises methodological questions related to equalization of exercise dose when comparing protocols. The present scoping review aims to identify in the literature the evidence for protocol equalization and the soundness of methods used for it. PubMed and Scopus databases were searched for original investigations comparing the effects of HIIT to MICT. A total of 2041 articles were identified, and 169 were included. Of these, 98 articles equalized protocols by utilizing energy-based methods or exercise volume (58 and 31 articles, respectively). No clear consensus for protocol equalization appears to have evolved over recent years. Prominent equalization methods consider the exercise dose (i.e., energy expenditure/production or total volume) in absolute values without considering the nonlinear nature of its relationship with duration. Exercises resulting from these methods induced maximal exertion in HIIT but low exertion in MICT. A key question is, therefore, whether exercise doses are best considered in absolute terms or relative to individual exercise maximums. If protocol equalization is accepted as an essential methodological prerequisite, it is hypothesized that comparison of program effects would be more accurate if exercise was quantified relative to intensity-related maximums.
Topics: Energy Metabolism; Exercise; Exercise Therapy; High-Intensity Interval Training; Humans
PubMed: 35564375
DOI: 10.3390/ijerph19094980 -
Journal of Physical Therapy Science Mar 2021[Purpose] For monitoring patients with knee osteoarthritis undergoing knee arthroplasty, the Timed Up and Go and maximum walking speed tests are commonly used. To...
[Purpose] For monitoring patients with knee osteoarthritis undergoing knee arthroplasty, the Timed Up and Go and maximum walking speed tests are commonly used. To provide appropriate peri-surgical rehabilitation, we evaluated the factors associated with postsurgical changes in Timed Up and Go and maximum walking speed results. [Participants and Methods] We enrolled 545 knee osteoarthritis patients undergoing either of the following knee arthroplasties: conventional total knee arthroplasty, minimally invasive total knee arthroplasty, and unicompartmental knee arthroplasty. Comfortable Timed Up and Go, maximum Timed Up and Go, and maximum walking speed were measured 2 weeks before and soon after surgery. Factors (gender, age, and surgical mode) that might influence changes in test results were evaluated by multiple regression analysis and a two-factor stratification diagram. [Results] Multiple regression analysis revealed that postsurgical changes in comfortable/maximum Timed Up and Go and maximum walking speed results were associated with age and surgical mode after adjustment for preoperative values. Two-factor diagrams showed that the older the patient, the greater was the slowdown in the Timed Up and Go test performed postoperatively. The levels of slowdown in the postoperative Timed Up and Go and maximum walking speed tests were the smallest in those who underwent conventional total knee arthroplasty, followed by those who underwent minimally invasive and unicompartmental knee arthroplasty. Among patients whose preoperative Timed Up and Go and maximum walking speed were slow, slowdown in Timed Up and Go was pronounced with age, and slowdown in maximum walking speed was higher in conventional total knee arthroplasty. [Conclusion] The changes in Timed Up and Go and maximum walking speed results 2 weeks after knee arthroplasty depended on age and surgical modes. These findings are relevant for the implementation of appropriate peri-surgical rehabilitation.
PubMed: 33814716
DOI: 10.1589/jpts.33.274 -
Sensors (Basel, Switzerland) Jul 2022(1) Background: Near-infrared spectroscopy (NIRS) is an innovative and non-invasive technology used to investigate muscular oxygenation. The aim of this study is to...
(1) Background: Near-infrared spectroscopy (NIRS) is an innovative and non-invasive technology used to investigate muscular oxygenation. The aim of this study is to assess the within- and between-session reliability of the NIRS Portamon (Artinis, Elst, Netherlands) device following three sets of induced muscle ischemia. (2) Methods: Depending on the experimental group (G1, G2 or G3), a cuff was inflated three times on the left upper arm to 50 mmHg (G1), systolic blood pressure (SBP) + 50 mmHg (G2) or 250 mmHg (G3). Maximum, minimum and reoxygenation rate values were assessed after each occlusion phase, using a Portamon device placed on the left brachioradialis. Reliability was assessed with intraclass correlation coefficient (ICC) value and ICC 95% confidence interval (CI-95%), coefficient of variation (CV) and standard error of measurement (SEM) (3) Results: Our results showed a good to excellent reliability for maximums and minimums within-session. However, the reoxygenation rate within sessions as well as measurements between sessions cannot predominantly show good reliability. (4) Conclusions: Multiple measurements of maximums and minimums within a single session appeared to be reliable which shows that only one measurement is necessary to assess these parameters. However, it is necessary to be cautious with a comparison of maximum, minimum and reoxygenation rate values between sessions.
Topics: Forearm; Humans; Ischemia; Muscle, Skeletal; Reproducibility of Results; Spectroscopy, Near-Infrared
PubMed: 35890846
DOI: 10.3390/s22145165 -
BMC Bioinformatics Oct 2018Many algorithms and programs are available for phylogenetic reconstruction of families of proteins. Methods used widely at present use either a number of distance-based...
BACKGROUND
Many algorithms and programs are available for phylogenetic reconstruction of families of proteins. Methods used widely at present use either a number of distance-based principles or character-based principles of maximum parsimony or maximum likelihood.
RESULTS
We developed a novel program, named PQ, for reconstructing protein and nucleic acid phylogenies following a new character-based principle. Being tested on natural sequences PQ improves upon the results of maximum parsimony and maximum likelihood. Working with alignments of 10 and 15 sequences, it also outperforms the FastME program, which is based on one of the distance-based principles. Among all tested programs PQ is proved to be the least susceptible to long branch attraction. FastME outperforms PQ when processing alignments of 45 sequences, however. We confirm a recent result that on natural sequences FastME outperforms maximum parsimony and maximum likelihood. At the same time, both PQ and FastME are inferior to maximum parsimony and maximum likelihood on simulated sequences. PQ is open source and available to the public via an online interface.
CONCLUSIONS
The software we developed offers an open-source alternative for phylogenetic reconstruction for relatively small sets of proteins and nucleic acids, with up to a few tens of sequences.
Topics: Algorithms; Phylogeny; Software
PubMed: 30314446
DOI: 10.1186/s12859-018-2399-4 -
Scientific Reports Aug 2023Rice is a staple food crop and is a major source of employment and income in the world. However, the attack of fungal disease poses a serious threat to the crop growth...
Rice is a staple food crop and is a major source of employment and income in the world. However, the attack of fungal disease poses a serious threat to the crop growth and productivity and leads toward yield loses. Therefore, current study was performed to evaluate the biocontrol potential of Lactobacillus and Weissella spp. on basmati rice against Rhizoctonia solani. Agar disc method was performed to evaluate the antifungal activity of both bacteria against R. solani. Petri plate and pot experiments were conducted to evaluate the growth promotion and biocontrol potential of both bacteria in Basmati rice under R. solani stress. Results indicated that maximum antifungal activity (82%) was recorded by Lactobacillus sp. Maximum phosphate solubilization and siderophore production was recorded by Weissella sp. In petri plate experiment, maximum root length, root fresh and dry weight (36%, 40% and 13%) was recorded by Weissella sp. and maximum shoot length and shoot fresh weight (99% and 107%) by Lactobacillus sp. In pot experiment, both bacteria enhanced the growth parameters of Basmati rice including root and shoot length, fresh and dry weight as well as no. of lateral roots. Application of Weissella sp. resulted in maximum increase (332% and 134%) in chlorophyll a and b content while Lactobacillus sp. + R. solani showed maximum (42%) carotenoid contents. Lactobacillus sp. + R. solani showed maximum increase in the proline (54%) and sugar contents (100%) while Lactobacillus sp. alone showed maximum (35%) soluble protein contents. Plant defense enzymes i-e SOD (400%), POD (25%), CAT (650%), PPO (14%) and PAL (124%) were notably increased by Weissella sp. + R. solani and Lactobacillus sp + R. solani. The Lactobacillus sp showed the best results in antifungal activity against R. solani and Weissella sp. showed the best results in production of defense enzymes in basmati rice against R. solani stress and can be suggested as the potent biocontrol agents for the rice crop.
Topics: Antifungal Agents; Chlorophyll A; Lactobacillus; Oryza; Weissella
PubMed: 37620521
DOI: 10.1038/s41598-023-41058-9 -
Frontiers in Physiology 2021We wanted to demonstrate the relationship between blood volume, cardiac size, cardiac output and maximum oxygen uptake ( O) and to quantify blood volume shifts during...
We wanted to demonstrate the relationship between blood volume, cardiac size, cardiac output and maximum oxygen uptake ( O) and to quantify blood volume shifts during exercise and their impact on oxygen transport. Twenty-four healthy, non-smoking, heterogeneously trained male participants (27 ± 4.6 years) performed incremental cycle ergometer tests to determine O and changes in blood volume and cardiac output. Cardiac output was determined by an inert gas rebreathing procedure. Heart dimensions were determined by 3D echocardiography. Blood volume and hemoglobin mass were determined by using the optimized CO-rebreathing method. The O ranged between 47.5 and 74.1 mL⋅kg⋅min. Heart volume ranged between 7.7 and 17.9 mL⋅kg and maximum cardiac output ranged between 252 and 434 mL⋅kg⋅min. The mean blood volume decreased by 8% (567 ± 187 mL, = 0.001) until maximum exercise, leading to an increase in [Hb] by 1.3 ± 0.4 g⋅dL while peripheral oxygen saturation decreased by 6.1 ± 2.4%. There were close correlations between resting blood volume and heart volume ( = 0.73, = 0.002), maximum blood volume and maximum cardiac output ( = 0.68, = 0.001), and maximum cardiac output and O ( = 0.76, < 0.001). An increase in maximum blood volume by 1,000 mL was associated with an increase in maximum stroke volume by 25 mL and in maximum cardiac output by 3.5 L⋅min. In conclusion, blood volume markedly decreased until maximal exhaustion, potentially affecting the stroke volume response during exercise. Simultaneously, hemoconcentrations maintained the arterial oxygen content and compensated for the potential loss in maximum cardiac output. Therefore, a large blood volume at rest is an important factor for achieving a high cardiac output during exercise and blood volume shifts compensate for the decrease in peripheral oxygen saturation, thereby maintaining a high arteriovenous oxygen difference.
PubMed: 34135772
DOI: 10.3389/fphys.2021.679232 -
Sports Medicine - Open 2015In this study, we examine the biomechanical advantage of combining localized vibrations to hamstring muscles involved in a traditional resistance training routine.
BACKGROUND
In this study, we examine the biomechanical advantage of combining localized vibrations to hamstring muscles involved in a traditional resistance training routine.
METHODS
Thirty-six male and female participants with at least 2 years of experience in resistance training were recruited from the German Sport University Cologne. The participants were randomized into two training groups: vibration training group (VG) and traditional training group (TTG). Both groups underwent a 4-week training phase, where each participant worked out at 70 % of the individual 1 repeat maximum (RM-maximum load capacity of a muscle for one lift to fatigue) (4 sets with 12 repetitions each). For participants in the VG group, local vibration was additionally applied directly to hamstring muscles during exercise. A 2-week examination phase preceded the pretests. After the pretests, the subjects underwent a prescribed training for 4 weeks. At the conclusion of the training, a 2-week detraining was imposed and then the study concluded with posttests and retest.
RESULTS
The measured parameters were maximum isometric force of the hamstrings and maximum range of motion and muscle tension at maximum knee angle. The study revealed a significant increase in maximum isometric force in both training groups (VG = 21 %, TTG = 14 %). However, VG groups showed an increase in their range of motion by approximately 2 %. Moreover, the muscle tension at maximum knee angle increased less in VG (approximately 35 %) compared to TG (approximately 46 %).
CONCLUSIONS
We conclude that segment-body vibrations applied in resistance training can offer an effective tool to increase maximum isometric force, compared to traditional training. The cause for these findings can be attributed to the additional local vibration stimulus.
PubMed: 26258006
DOI: 10.1186/s40798-015-0022-z -
Scientific Reports Jun 2022In order to explore the thermal load change of the diesel engine piston under transitional conditions, and the influence of the position of cooling gallery on the heat...
In order to explore the thermal load change of the diesel engine piston under transitional conditions, and the influence of the position of cooling gallery on the heat transfer characteristics of the piston. An off-road high-pressure common-rail diesel engine is chosen as the research object. The sequence coupling method is used to establish the fluid-solid coupling heat transfer simulation model of the piston-gallery under the transition conditions of cold start, urgent acceleration and rapid deceleration. The Pareto optimization algorithm is introduced to optimize the position of the cooling gallery to reduce the maximum temperature and maximum thermal stress of the piston. The results show that the maximum temperature of the piston can be reduced by reducing the distance between the cooling gallery and the throat area under the maximum torque condition, and that the maximum thermal stress of the piston can be reduced by reducing the distance between the cooling gallery and the throat area or by increasing the distance between the cooling gallery and the ring area. Compared with the original design, the maximum temperature of Design A decreases by 1.28 °C while the maximum thermal stress decreases by 2.07 MPa. The maximum temperature and maximum thermal stress of Design B decreases by 0.22 °C and 0.5 MPa, respectively. The maximum thermal stress of Design C decreases by 2.67 MPa when the maximum temperature increases by 1.15 °C. The maximum change in temperature of the three typical designs and the original design of the piston throat under cold start, urgent acceleration and rapid deceleration conditions reached 207.29 °C, 136.78 °C and 9.89 °C, and the maximum change of thermal stress reached 8.62 MPa, 20.43 MPa, 4.08 MPa, respectively. The maximum change in temperature of the piston first ring groove under cold start, urgent acceleration and rapid deceleration conditions reached 172.00 °C, 83.52 °C and 7.36 °C, and the maximum change in thermal stress reached 22.96 MPa, 43.10 MPa, 5.72 MPa, respectively. The conclusions obtained can provide boundary conditions for further study of the thermal load change law of the same type of pistons, and also provide a theoretical basis for diesel engine piston structure optimization and the performance improvement.
PubMed: 35665771
DOI: 10.1038/s41598-022-13357-0