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Scientific Reports Oct 2023Surgical sterilization or neutering of dogs is a commonly performed procedure in veterinary practices in many countries. In recent decades, concerns have been raised...
Surgical sterilization or neutering of dogs is a commonly performed procedure in veterinary practices in many countries. In recent decades, concerns have been raised regarding possible side effects of neutering, including increased risk of certain neoplastic, musculoskeletal and endocrinological conditions. Considering that age serves as a significant confounding factor for some of these conditions, evaluating longevity statistics could provide valuable insights into the impact of neutering. The aim of this study was to compare longevity between neutered and sexually intact male and female Rottweilers, using electronic patient records collected by the VetCompass Australia database. Male and female Rottweilers neutered before 1 year of age (n = 207) demonstrated an expected lifespan 1.5 years and 1 year shorter, respectively, than their intact counterparts (n = 3085; p < 0.05). Broadening this analysis to include animals neutered before the age of 4.5 years (n = 357) produced similar results.
Topics: Humans; Dogs; Male; Female; Animals; Infant; Child, Preschool; Longevity; Ovariectomy; Sterilization, Reproductive; Australia; Dog Diseases
PubMed: 37857755
DOI: 10.1038/s41598-023-45128-w -
Menopause International Mar 2009
Topics: Aging; Cost-Benefit Analysis; Estrogen Replacement Therapy; Female; Humans; Menopause; Ovariectomy
PubMed: 19237612
DOI: 10.1258/mi.2008.009012 -
Oxidative Medicine and Cellular... 2019Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy...
Hormone replacement therapy (HRT) can alleviate estrogen deficiency symptoms especially during menopause. The present study aimed at investigating the effect of soy isoflavones as HRT on immunological and bone health-related parameters with a special focus on the interactions between immunological status and metabolism. Thirty healthy cyclic female Wistar rats were used in this experiment. Ten females were sham-operated, and 20 females were subjected to ovariectomy. Overiectomized (OVX) female rats were randomly divided into 2 groups: the control group (G1, OVX/casein) was fed a casein-based diet, and the second group (G2, OVX/soy) was fed a high soy isoflavone diet. Both groups were compared to a sham-operated group (G3, sham/casein). Treatments continued for 7 weeks. Feed intake, weight gain, and lymphoid organ relative weights were recorded. Some metabolic, immunological, and bone health-related parameters were measured. Moreover, nitric oxide (NO), malondialdehyde (MDA), and total antioxidant capacity (TAC) were determined. Bone histopathology and immunohistochemistry to estrogen receptor alpha (ER) were done. Feeding soy to OVX females reduced feed intake, weight gain, relative lymphoid organ weight, and T-lymphocytes transformation. Soy isoflavone administration normalized nearly all metabolic and immunological parameters to a level comparable to the sham group via oxidative stress amelioration and bone ER promotion. Soy isoflavones seemed to be good HRT in estrogen deprivation which modulated the appetite, weight gain, lipid profile, proinflammation, and bone turnover.
Topics: Animals; Antioxidants; Estrogens; Female; Isoflavones; Ovariectomy; Rats; Rats, Wistar; Glycine max
PubMed: 30733850
DOI: 10.1155/2019/5713606 -
Schweizer Archiv Fur Tierheilkunde Jun 2010The necessity, and in particular the timing of gonadectomy in dogs is still controversial. This is mainly because gonadectomy confers a mixture of benefits and adverse... (Review)
Review
The necessity, and in particular the timing of gonadectomy in dogs is still controversial. This is mainly because gonadectomy confers a mixture of benefits and adverse effects depending on age at neutering, breed and sex. On advising a client on gonadectomy, the frequency and consequences of the pros and cons should be balanced.
Topics: Animals; Cognition; Dogs; Female; Hysterectomy; Male; Orchiectomy; Ovariectomy; Quality of Life; Risk Factors
PubMed: 20533199
DOI: 10.1024/0036-7281/a000063 -
Women's Health (London, England) Sep 2009Bilateral oophorectomy at the time of hysterectomy for benign disease is commonly practiced in order to prevent the subsequent development of ovarian cancer or other... (Review)
Review
Bilateral oophorectomy at the time of hysterectomy for benign disease is commonly practiced in order to prevent the subsequent development of ovarian cancer or other ovarian pathology that might require additional surgery. At present, bilateral oophorectomy is performed in 78% of women aged between 45 and 64 years having a hysterectomy, and a total of approximately 300,000 prophylactic oophorectomies are performed in the USA every year. Estrogen deficiency resulting from pre- and post-menopausal oophorectomies has been associated with higher risks of coronary heart disease, stroke, hip fracture, Parkinsonism, dementia, cognitive impairment, depression and anxiety in many studies. While ovarian cancer accounts for 14,800 deaths per year in the USA, coronary heart disease accounts for 350,000 deaths per year. In addition, 100,000 cases of dementia may be attributable annually to prior bilateral oophorectomy. At present, observational studies suggest that bilateral oophorectomy may do more harm than good. In women who are not at high risk of developing ovarian or breast cancer, removing the ovaries at the time of hysterectomy should be approached with caution.
Topics: Affect; Decision Making; Female; Health Status; Hip Fractures; Humans; Hysterectomy; Middle Aged; Osteoporosis; Ovariectomy; Ovary; Quality of Life; Risk Assessment; Sexuality; Time Factors; Treatment Outcome; Women's Health
PubMed: 19702455
DOI: 10.2217/whe.09.42 -
Journal of Veterinary Science 2015This study was conducted to evaluate the feasibility and therapeutic safety of laparoendoscopic single-site ovariectomy (LESS-OVE) and 3-portal laparoscopic ovariectomy... (Comparative Study)
Comparative Study
This study was conducted to evaluate the feasibility and therapeutic safety of laparoendoscopic single-site ovariectomy (LESS-OVE) and 3-portal laparoscopic ovariectomy (Lap-OVE) in dogs. Ten female mixed breed dogs were included in the study. Dogs were divided into group 1 (LESS-OVE; n = 5) and group 2 (Lap-OVE; n = 5). All procedures were performed by laparoscopic-skilled surgeons, and the anesthetic protocol was the same for all patients. In both groups, the ovarian vascular pedicle and ligaments were transected using a bipolar vessel sealer/divider device. The mean total surgical time was slightly longer in LESS-OVE (36.6 ± 3.5 min) than Lap-OVE (32.0 ± 3.0 min); however, the differences were not significant. Perioperative complications were not reported in any group. Both laparoscopic techniques were shown to be equally feasible and safe for patients. However, surgeons found LESS-OVE to require more skill than Lap-OVE. Therefore, additional studies should be conducted to evaluate this novel approach in clinical veterinary practice, and a proper laparoscopic training program for veterinary surgeons should be developed.
Topics: Animals; Dogs; Female; Laparoscopy; Ovariectomy
PubMed: 26119164
DOI: 10.4142/jvs.2015.16.4.525 -
Frontiers in Endocrinology 2022Yin Huo Tang (YHT), a traditional Chinese herbal formula, is effectively used for the clinical treatment of menopause-like symptoms in China. This study aimed to...
BACKGROUND
Yin Huo Tang (YHT), a traditional Chinese herbal formula, is effectively used for the clinical treatment of menopause-like symptoms in China. This study aimed to investigate its efficacy on menopause-like symptoms in mice using behavioral tests and histopathological assessment, and to determine its possible mechanism of action based on network pharmacology.
METHODS
Liquid chromatography-mass spectrometry (LC-MS) technology was used to identify the potential active ingredients of YHT. In mice, menopause-like symptoms were induced by combination of bilateral ovariectomy and empty bottle stimulation. The mice were then treated with the YHT aqueous extract for three weeks. Behavior, sleep state, body weight, organ index, and histomorphology were analyzed separately. Additionally, network pharmacology and molecular docking were used to predict the mechanisms underlying the action of YHT. Finally, serum estradiol was quantified to preliminarily verify the results of network pharmacology.
RESULTS
YHT not only improved the behavior of mice (attack and explore behavior reduced; modify behavior increased) but also ameliorated the sleep state (sleep time increased and incubation time reduced). YHT reduced body weight, increased uterine weight, and improved the histomorphology of some organs. Network pharmacology and molecular docking analyses revealed that the estrogen signaling pathway might play a key role in attenuating menopause-like symptoms. Furthermore, YHT treatment reversed the reduction in serum estradiol levels.
CONCLUSIONS
YHT alleviates menopause-like symptoms in a mouse model, providing a rationale for using it as a potential therapeutic strategy.
Topics: Humans; Female; Animals; Mice; Molecular Docking Simulation; Ovariectomy; Menopause; Estrogens; Estradiol
PubMed: 36339416
DOI: 10.3389/fendo.2022.994642 -
Journal of Feline Medicine and Surgery Feb 2020The aim of this study was to prospectively compare perioperative pain score and wound parameters, inclusive of postoperative swelling and erythema, between flank and...
OBJECTIVES
The aim of this study was to prospectively compare perioperative pain score and wound parameters, inclusive of postoperative swelling and erythema, between flank and midline ovariectomy (OVE) in cats, performed by final-year veterinary students.
METHODS
Healthy cats presented for routine OVE were randomly assigned to either the midline or flank group after owner consent to participate in the study. Perioperative protocols were standardised for both groups. Clinical data were collected prior to surgery, intraoperatively, at 1 h postoperatively, at the time of discharge, and at 3 and 10 day postoperative re-examination appointments. Data recorded included duration of surgery and anaesthesia, intraoperative complications, Feline Acute Pain Scale (FAPS) scores, a simple descriptive scale of reaction to wound palpation (SDS), a dynamic and interactive visual analogue scale assessment of pain (DIVAS), and both a simple descriptive scale (SDS) and a visual analogue scale (VAS) of surgical wound swelling.
RESULTS
Thirty-eight cats received a flank OVE and 37 received a midline OVE. Duration of surgery, duration of anaesthesia and intraoperative complications did not vary significantly between the two groups. Cats in both groups had significantly higher FAPS scores after surgery ( = 0.0002), with cats receiving a flank OVE having significantly higher pain scores compared with a midline OVE at 1 h postoperatively ( = 0.0004) and at discharge ( = 0.002). Swelling of the surgical wound (SDS) was significantly higher in cats receiving a midline OVE at the time of discharge ( = 0.048), as well as at the 3 day ( <0.0001) and 10 day ( = 0.001) postoperative re-examinations. FAPS scores were significantly higher in cats receiving a midline OVE at the 3 day ( = 0.016) and 10 day re-examinations ( = 0.045). No cats in either group suffered a wound breakdown or infection.
CONCLUSIONS AND RELEVANCE
Our study does not support advocating a preferred surgical approach for feline OVE within a teaching environment.
Topics: Animals; Cat Diseases; Cats; Female; Ovariectomy; Pain, Postoperative; Prospective Studies; Surgical Wound
PubMed: 30950672
DOI: 10.1177/1098612X19837038 -
Journal of Orthopaedic Surgery and... Mar 2023To explore the difference in the protective effects of intraperitoneal injection of exogenous melatonin of daytime or nighttime on bone loss in ovariectomized (OVX) rats.
OBJECTIVE
To explore the difference in the protective effects of intraperitoneal injection of exogenous melatonin of daytime or nighttime on bone loss in ovariectomized (OVX) rats.
METHODS
After bilateral ovariectomy and sham surgery, 40 rats were randomly divided into four groups: sham operation group (Sham), ovariectomy (OVX), and daytime melatonin injection group (OVX + DMLT, 9:00, 30 mg/kg/d) and nighttime injection of melatonin (OVX + NMLT, 22:00, 30 mg/kg/d). After 12 weeks of treatment, the rats were sacrificed. The distal femur, blood and femoral marrow cavity contents were saved. The rest of the samples were tested by Micro-CT, histology, biomechanics and molecular biology. Blood was used for bone metabolism marker measurements. CCK-8, ROS, and Cell apoptosis are performed using MC3E3-T1 cells.
RESULTS
Compared with treatment at night, the bone mass of the OVX rats was significantly increased after the daytime administration. All microscopic parameters of trabecular bone increased, only Tb.Sp decreased. Histologically, the bone microarchitecture of the OVX + DMLT was also more dense than the bone microarchitecture of the OVX + LMLT. In the biomechanical experiment, the femur samples of the day treatment group were able to withstand greater loads and deformation. In molecular biology experiments, bone formation-related molecules increased, while bone resorption-related molecules decreased. After treatment with melatonin administration at night, the expression of MT-1β was significantly decreased. In cell experiments, the MC3E3-T1 cells treated with low-dose MLT had higher cell viability and greater efficiency in inhibiting ROS production than the MC3E3-T1 cells treated with high-dose MLT, which in turn more effectively inhibited apoptosis.
CONCLUSION
Daytime administration of melatonin acquires better protective effects on bone loss than night in OVX rats.
Topics: Female; Rats; Animals; Humans; Osteoporosis; Melatonin; Reactive Oxygen Species; Bone Diseases, Metabolic; Bone Density; Femur; Ovariectomy
PubMed: 36949499
DOI: 10.1186/s13018-023-03695-8 -
Biomedicine & Pharmacotherapy =... Feb 2022Osteoporosis is a common disease characterized by skeletal fragility and microarchitectural deterioration. However, existing conventional drugs exhibit limited efficacy...
Osteoinductive activity of bisdemethoxycurcumin and its synergistic protective effect with human amniotic mesenchymal stem cells against ovariectomy-induced osteoporosis mouse model.
Osteoporosis is a common disease characterized by skeletal fragility and microarchitectural deterioration. However, existing conventional drugs exhibit limited efficacy and can elicit severe adverse effects; moreover, and novel stem cell-based therapies have not exhibited sufficient therapeutic efficacy. Our hypothesis is that an appropriate osteogenic inducer may improve their therapeutic efficacy. In this study, we found that bisdemethoxycurcumin (BDMC) stimulates the differentiation of human amniotic mesenchymal stem cells (hAMSCs) into osteoblasts without inducing cytotoxicity. Here BDMC enhances calcium deposition in hAMSCs, while promoting the expression of early and late markers of osteoblast differentiation, including ALP, runt-related transcription factor 2, osterix, COL1-α1, osteocalcin, and osteopontin at the transcriptional and translational levels. Mechanistically, BDMC was found to activate the JAK2/STAT3 pathway; whereas AG490 (JAK2/STAT3 pathway inhibitor) inhibited BDMC functioning. Subsequently, we found that the combinatorial therapy of BDMC and hAMSC had a positive synergistic effect on osteoporotic mouse model induced by bilateral ovariectomy, including inhibiting bone loss and bone resorption and improving bone micro-architecture. Moreover, BDMC inhibited production of the bone resorption markers C-terminal telopeptide of type I collagen, and tartrate resistant acid phosphatase, while promoting serum levels of bone formation markers OCN, and procollagen I N-terminal propeptide. BDMC also improved liver and kidney function in osteoporotic mouse model. Collectively, BDMC improved osteoporosis by enhancing hAMSC osteogenesis and exhibited a protective effect on liver and kidney function in an osteoporotic mouse model. Hence, BDMC may serve as an effective adjuvant, and combined therapy with hAMSCs is a promising new approach toward osteoporosis treatment.
Topics: Animals; Diarylheptanoids; Female; Humans; Mesenchymal Stem Cells; Mice; Osteogenesis; Osteoporosis; Ovariectomy
PubMed: 35062070
DOI: 10.1016/j.biopha.2021.112605