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Journal of Biomedical Optics Jun 2013The importance of dermatological noninvasive imaging techniques has increased over the last decades, aiming at diagnosing nonmelanoma skin cancer (NMSC). Technological... (Review)
Review
The importance of dermatological noninvasive imaging techniques has increased over the last decades, aiming at diagnosing nonmelanoma skin cancer (NMSC). Technological progress has led to the development of various analytical tools, enabling the in vivo/in vitro examination of lesional human skin with the aim to increase diagnostic accuracy and decrease morbidity and mortality. The structure of the skin layers, their chemical composition, and the distribution of their compounds permits the noninvasive photodiagnosis of skin diseases, such as skin cancers, especially for early stages of malignant tumors. An important role in the dermatological diagnosis and disease monitoring has been shown for promising spectroscopic and imaging techniques, such as fluorescence, diffuse reflectance, Raman and near-infrared spectroscopy, optical coherence tomography, and confocal laser-scanning microscopy. We review the use of these spectroscopic techniques as noninvasive tools for the photodiagnosis of NMSC.
Topics: Carcinoma, Basal Cell; Dermoscopy; Diagnostic Imaging; Equipment Design; Humans; Light; Microscopy, Confocal; Nitrogen; Skin; Skin Neoplasms; Spectrometry, Fluorescence; Spectrophotometry; Spectroscopy, Near-Infrared; Spectrum Analysis, Raman; Tomography, Optical Coherence; Tyrosine
PubMed: 23748702
DOI: 10.1117/1.JBO.18.6.061221 -
Cancer Nov 2003Recent advances in fiber optics, sources and detectors, imaging, and computer-controlled instrumentation have stimulated a period of unprecedented growth in the... (Review)
Review
Recent advances in fiber optics, sources and detectors, imaging, and computer-controlled instrumentation have stimulated a period of unprecedented growth in the development of photonics technologies for a wide variety of diagnostic and therapeutic clinical applications. These include the application of quantitative optical spectroscopy and imaging for the detection of precancerous lesions in the uterine cervix, a topic of interest at the Second International Conference on Cervical Cancer, which was held April 11-14, 2002. Investigators have applied the Littenberg method of emerging technology assessment to new optical methods used to detect cervical neoplasia. Currently, such technologies as fluorescence spectroscopy (the combination of fluorescence and diffuse reflectance spectroscopy), tri-modal spectroscopy, and light-scattering spectroscopy that probe the spectral characteristics of tissue are being investigated. Optical technologies that create images of subcellular structure without biopsy subsequent to pathology that currently are under investigation include in vivo confocal imaging and optical coherence tomography. Numerous small studies have demonstrated the potential of these optical technologies. What remains to be elucidated are the fundamental biophysical origins of variations in remitted optical signals between normal and dysplastic tissue. Large multicenter randomized controlled trials are needed to confirm the detection and imaging capabilities of optical technology. Furthermore, the development of contrast agents that could boost detection with these technologies is needed, and basic biologic characterization of signals should be pursued. Applying the Littenberg assessment will help ensure that superior, not simply alternative, technologies are implemented.
Topics: Cervix Uteri; Female; Humans; Microscopy, Confocal; Precancerous Conditions; Spectrometry, Fluorescence; Spectrum Analysis; Tomography, Optical Coherence; Uterine Cervical Neoplasms
PubMed: 14603538
DOI: 10.1002/cncr.11678 -
Journal of Biomedical Optics 2014A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk...
A new imaging approach, structured light scatteroscopy (SLS), is demonstrated, which offers rapid wide-field imaging of microscopic morphological variations in bulk tissue surfaces. Elastic scattering of light offers exquisite sensitivity to ultrastructural changes at multiple size scales ranging from nanometers to millimeters, but in bulk tissues the confounding effects of molecular absorption and strong multiple scattering of light often lead to a dramatic reduction in scatter contrast and specificity. It is demonstrated that the SLS using structured high spatial frequency illumination and detection to probe the tissue achieves direct, absorption-independent, high-resolution maps of the scattering response. The scattering response is observed to be dependent on both the wavelength and spatial frequency of choice, indicating a potential for multiscale probing of ultrastructural changes in superficial tissue layers. This methodology can be easily applied in most wide-field imaging systems.
Topics: Absorption, Physicochemical; Animals; Blood Physiological Phenomena; Cattle; Light; Muscle, Skeletal; Phantoms, Imaging; Scattering, Radiation; Spectrum Analysis
PubMed: 25057960
DOI: 10.1117/1.JBO.19.7.070504 -
Annual Review of Physical Chemistry 2011Molecular diffusion and transport processes are fundamental in physical, chemical, and biological systems. Current approaches to measuring molecular transport in cells... (Review)
Review
Molecular diffusion and transport processes are fundamental in physical, chemical, and biological systems. Current approaches to measuring molecular transport in cells and tissues based on perturbation methods, e.g., fluorescence recovery after photobleaching, are invasive; single-point fluctuation correlation methods are local; and single-particle tracking requires the observation of isolated particles for relatively long periods of time. We discuss here the detection of molecular transport by exploiting spatiotemporal correlations measured among points at large distances (>1 μm). We illustrate the evolution of the conceptual framework that started with single-point fluorescence fluctuation analysis based on the transit of fluorescent molecules through a small volume of illumination. This idea has evolved to include the measurement of fluctuations at many locations in the sample using microscopy imaging methods. Image fluctuation analysis has become a rich and powerful technique that can be used to extract information about the spatial distribution of molecular concentration and transport in cells and tissues.
Topics: Biological Transport; Diffusion; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescent Dyes; Image Interpretation, Computer-Assisted; Kinetics; Models, Molecular; Spectrometry, Fluorescence; Spectrum Analysis
PubMed: 21219151
DOI: 10.1146/annurev-physchem-032210-103424 -
Molecules (Basel, Switzerland) Feb 2017n/a.
n/a.
Topics: Animals; Biological Science Disciplines; Humans; Spectrum Analysis
PubMed: 28208823
DOI: 10.3390/molecules22020278 -
Analytical Sciences : the International... 2021
Topics: Mass Spectrometry; Single-Cell Analysis; Spectrum Analysis
PubMed: 34897178
DOI: 10.2116/analsci.highlights2112 -
Fa Yi Xue Za Zhi Oct 2022Hyperspectral imaging technology can obtain the spatial and spectral three-dimensional imaging of substances simultaneously, and obtain the unique continuous...
Hyperspectral imaging technology can obtain the spatial and spectral three-dimensional imaging of substances simultaneously, and obtain the unique continuous characteristic spectrum of substances in a wide spectrum range at a certain spatial resolution, which has outstanding advantages in the fine classification and identification of biological substances. With the development of hyperspectral imaging technology, a large amount of data has been accumulated in the exploration of data acquisition, image processing and material inspection. As a new technology means, hyperspectral imaging technology has its unique advantages and wide application prospects. It can be combined with the common biological physical evidence of blood (stains), saliva, semen, sweat, hair, nails, bones, etc., to achieve rapid separation, inspection and identification of substances. This paper introduces the basic theory of hyperspectral imaging technology and its application in common biological evidence examination research and analyzes the feasibility and development of biological evidence testing and identification, in order to provide a theoretical basis for the development of new technology and promote hyperspectral imaging technology in related biological examination, to better serve the forensic practice.
Topics: Spectrum Analysis; Hyperspectral Imaging; Forensic Medicine; Blood Stains; Technology
PubMed: 36727181
DOI: 10.12116/j.issn.1004-5619.2021.510904 -
Analytical Sciences : the International... 2016Efficient environment protection and human safety require high-throughput analysis techniques for pollutants or toxicants for large sample sets. State-of-the-art HPLC... (Review)
Review
Efficient environment protection and human safety require high-throughput analysis techniques for pollutants or toxicants for large sample sets. State-of-the-art HPLC and GC coupled to various detecting strategies offer excellent sensitivity and selectivity, though they are quite time-extensive (2 - 3 samples/h or less when sample preparation is involved). Efforts are made towards screening techniques with high sample throughputs simultaneously providing detection limits below the maximum contaminant levels for the analyte. However, such approaches frequently sacrifice the selectivity or sensitivity (or just give a yes/no response). In this review, we demonstrate thermal-lens spectrometry and microscopy as highly sensitive spectrometric techniques in combination with flow-injection analysis (FIA) and microfluidic FIA along with lab-on-a-chip chemistry for fast screening (several samples/h and up to 20 samples/min) exemplified by organophosphates and carbamates as neurotoxigenic compounds. Various approaches to determining other topical toxicants, like microcystin and cyanopigments as its indicators, allergens, and carcinogenic chromate, are also discussed.
Topics: Carbamates; Environmental Pollutants; Equipment Design; Flow Injection Analysis; Hazardous Substances; Lab-On-A-Chip Devices; Microfluidic Analytical Techniques; Microscopy; Neurotoxins; Organophosphates; Sensitivity and Specificity; Spectrum Analysis; Temperature
PubMed: 26753701
DOI: 10.2116/analsci.32.23 -
Journal of Biomedical Optics Jun 2013Carotenoids are important substances for human skin due to their powerful antioxidant properties in reaction of neutralization of free radicals and especially reactive... (Comparative Study)
Comparative Study Review
Carotenoids are important substances for human skin due to their powerful antioxidant properties in reaction of neutralization of free radicals and especially reactive oxygen species, including singlet oxygen. Concentration of carotenoids in the skin could mirror the current redox status of the skin and should be investigated in vivo. Optical methods are ideally suited for determination of carotenoids in mammalian skin in vivo as they are both noninvasive and quick. Four different optical methods could be used for in vivo measurement of carotenoids in the human or animal skin: (1) resonance Raman spectroscopy; (2) Raman microscopy; (3) reflection spectroscopy; (4) skin color measurements. The advantages, shortcomings, and limitations of the above-mentioned optical methods are discussed.
Topics: Animals; Carotenoids; Dermatology; Humans; Microscopy; Optical Phenomena; Skin; Skin Pigmentation; Spectrum Analysis; Spectrum Analysis, Raman
PubMed: 23426582
DOI: 10.1117/1.JBO.18.6.061230 -
Molecular Neurodegeneration May 2018Neurodegenerative diseases are a growing burden in modern society, thus crucially calling for the development of accurate diagnostic strategies. These diseases are...
Neurodegenerative diseases are a growing burden in modern society, thus crucially calling for the development of accurate diagnostic strategies. These diseases are currently incurable, a fact which has been attributed to their late diagnosis, after brain damage has already become widespread. An earlier and improved diagnosis is necessary for the enrolment of patients into clinical trials and can pave the way for the development of therapeutic tactics. Novel analytical techniques, such as mass spectrometry and vibrational spectroscopy, have been able to successfully detect and characterise neurodegenerative disorders. It is critical to globally support and make use of innovative basic research and techniques, which could ultimately lead to the creation of a cost-effective diagnostic test. Minimally invasive samples, such as biological fluids, have also been shown to reveal information for these diseases; utilising them could simplify sample collection/analysis and be more preferable to patients.
Topics: Humans; Neurodegenerative Diseases; Spectrum Analysis
PubMed: 29716629
DOI: 10.1186/s13024-018-0252-x