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Anais Da Academia Brasileira de Ciencias 2022Kavain is one of the main kavalactones of Piper methysticum (Piperaceae) with anxiolytic, analgesic, and antioxidant activities. Therefore, the aim of the study was to...
Kavain is one of the main kavalactones of Piper methysticum (Piperaceae) with anxiolytic, analgesic, and antioxidant activities. Therefore, the aim of the study was to evaluate the cytotoxic, mutagenic, and antimutagenic potential of kavain in Allium cepa cells. Roots of A. cepa were transferred to the negative (2% acetone) and positive (10 µg/mL of Methylmethanesulfonate, MMS) controls and to the concentrations of kavain (32, 64 and 128 µg/mL) for 48 h. A total of 5,000 meristematic cells were analyzed under an optical microscope to determine the mitotic index, mean number of chromosomal alterations and percentage of damage reduction. Data were analyzed by Kruskal-Wallis test (p <0.05). All concentrations of kavain were not cytotoxic and did not show significant chromosomal changes when compared to 2% acetone. Kavain showed a cytoprotective effect in the pre (128 μg/mL) and in the post-treatment (32 and 64 μg/mL) and reduced damage against the mutagenic action of MMS in all concentrations of the pre and simultaneous and at the highest of post (128 μg/mL). Kavain promoted a significant reduction in micronuclei, nuclear buds and chromosomal losses in relation to MMS. The observed data indicate the importance of kavain for the inhibition of damage and chemoprevention.
Topics: Acetone; Chromosome Aberrations; Meristem; Mutagens; Onions; Plant Roots; Pyrones
PubMed: 35703688
DOI: 10.1590/0001-3765202220200520 -
International Journal of Environmental... Dec 2022Based on the porous carbon material from citric acid residue, catalysts of different Ce-Mn ratios were prepared with incipient-wetness impregnation (IWI) to delve into...
Based on the porous carbon material from citric acid residue, catalysts of different Ce-Mn ratios were prepared with incipient-wetness impregnation (IWI) to delve into their acetone-degrading performance and relevant mechanisms. When the Ce-Mn molar ratio is 0.8, the prepared catalyst Ce-Mn/AC shows abundant and uniformly dispersed Mn and Ce particles on the surface. The content of Mn and Ce on the Ce-Mn/AC surface reaches 5.64% and 0.75%, respectively. At the acetone concentration of 238 mg/m (100 ppm), the laws of acetone degradation in different catalysts at different catalyzing temperatures and with various oxygen concentrations were studied, and we found that the rate of acetone degradation by Ce-Mn/AC can exceed 90% at 250 °C. Cerium oxide and manganese oxide are synergistic in the catalytic degradation of acetone. Adding cerium to manganese-based catalysts can increase the oxygen migration rate in the catalysts and thus raise the reduction rate of lattice oxygen in manganese oxide. The results offer new ideas and approaches for the efficient and comprehensive utilization of bio-fermentation by-products, and for the development of cheap and high degradation performance catalysts for acetone.
Topics: Temperature; Acetone; Oxides; Oxygen; Catalysis
PubMed: 36554760
DOI: 10.3390/ijerph192416879 -
Journal of Magnetic Resonance Imaging :... Dec 2017To propose and evaluate an acetone-D O phantom that has an extended range of apparent diffusion coefficient (ADC) for quantitative diffusion magnetic resonance imaging...
PURPOSE
To propose and evaluate an acetone-D O phantom that has an extended range of apparent diffusion coefficient (ADC) for quantitative diffusion magnetic resonance imaging (MRI), as well as to compare its properties to previously described water-based phantoms.
MATERIALS AND METHODS
The proposed acetone-D O, and previously described sucrose water solution and polyvinylpyrrolidone (PVP) water solution phantoms, were constructed in a number of concentrations between 0% and 50%. At 1.5T field strength, diffusion-weighted MR spectroscopy (DW-MRS), based on a point-resolved spectroscopy (PRESS) acquisition, nondiffusion-weighted stimulated echo acquisition mode (STEAM)-MRS, and diffusion-weighted echo-planar imaging (DW-EPI) were used to evaluate each phantom. The MR spectra, diffusion-weighted signal decay pattern, tunability of ADC, and ADC range of each phantom were all evaluated.
RESULTS
When placed in an ice-water bath, all phantoms provided desirable signal properties, including single-peak signal with Gaussian diffusion and tunable ADC. At 0°C, however, water-based phantoms had ADC limited to less than 1.1·10 mm ·s (0.2-1.1·10 mm ·s ), while the proposed acetone-based phantom had ADC values spanning a wider range (0.6-3.5·10 mm ·s ).
CONCLUSION
The proposed acetone-D O phantom provided desirable signal properties over a wide range of ADCs with temperature controlled using an ice-water bath.
LEVEL OF EVIDENCE
2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2017;46:1683-1692.
Topics: Acetone; Diffusion Magnetic Resonance Imaging; Phantoms, Imaging
PubMed: 28432796
DOI: 10.1002/jmri.25727 -
Microbiological Reviews Dec 1986
Review
Topics: Acetone; Butanols; Clostridium; Fermentation
PubMed: 3540574
DOI: 10.1128/mr.50.4.484-524.1986 -
Biosensors Jun 2022Breath sensor technology can be used in medical diagnostics. This study aimed to build a device to measure the level of hydrogen sulfide, ammonia, acetone and alcohol in...
Breath sensor technology can be used in medical diagnostics. This study aimed to build a device to measure the level of hydrogen sulfide, ammonia, acetone and alcohol in exhaled breath of patients as well as healthy individuals. The purpose was to determine the efficacy of these gases for detection of obstructive lung disease. This study was conducted on a total of 105 subjects, where 60 subjects were patients of obstructive lung disease and 45 subjects were healthy individuals. Patients were screened by means of the Pulmonary Function Test (PFT) by a pulmonologist. The gases present in the exhaled breath of all subjects were measured. The level of ammonia (32.29 ± 20.83 ppb), (68.83 ± 35.25 ppb), hydrogen sulfide (0.50 ± 0.26 ppm), (62.71 ± 22.20 ppb), and acetone (103.49 ± 35.01 ppb), (0.66 ± 0.31 ppm) in exhaled breath were significantly different (p < 0.05) between obstructive lung disease patients and healthy individuals, except alcohol, with a p-value greater than 0.05. Positive correlation was found between ammonia w.r.t Forced Expiratory Volume in 1 s (FEV1) (r = 0.74), Forced Vital Capacity (FVC) (r = 0.61) and Forced Expiratory Flow (FEF) (r = 0.63) and hydrogen sulfide w.r.t FEV1 (r = 0.54), FVC (r = 0.41) and FEF (r = 0.37). Whereas, weak correlation was found for acetone and alcohol w.r.t FEV1, FVC and PEF. Therefore, the level of ammonia and hydrogen sulfide are useful breath markers for detection of obstructive lung disease.
Topics: Acetone; Ammonia; Breath Tests; Humans; Hydrogen Sulfide; Lung Diseases, Obstructive
PubMed: 35735555
DOI: 10.3390/bios12060409 -
Cells Jan 2022Furfural acetone (FAc) is a promising alternative to currently available nematicides, and it exhibits equivalent control efficiency on root-knot nematodes with...
Furfural acetone (FAc) is a promising alternative to currently available nematicides, and it exhibits equivalent control efficiency on root-knot nematodes with avermectin in fields. However, its effect on the reproduction of root-knot nematode is poorly understood. In this study, the natural metabolite FAc was found to exhibit reproductive toxicity on and . The number of germ cells of was observed to decrease after exposure to FAc, with a reduction of 59.9% at a dose of 200 mg/L. FAc in various concentrations induced the germ-cell apoptosis of , with an increase over six-fold in the number of apoptotic germ cells at 200 mg/L. These findings suggested that FAc decreased the brood size of nematode by inducing germ-cell apoptosis. Moreover, FAc-induced germ-cell apoptosis was suppressed by the mutation of gene , , , , , , or . The expression of genes , , and in was increased significantly after FAc treatment. Taken together, these results indicate that nematode exposure to FAc might inflict DNA damage through protein SPO-11, activate CEP-1 and EGL-1, and induce the core apoptosis pathway to cause germ-cell apoptosis, resulting in decreased brood size of .
Topics: Acetone; Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Furaldehyde; Reproduction; Tylenchoidea
PubMed: 35159211
DOI: 10.3390/cells11030401 -
Nutrition & Diabetes Sep 2018An exploratory study was performed on eight healthy volunteers to assess how short-term changes in energy balance and dietary carbohydrate content impact breath acetone...
An exploratory study was performed on eight healthy volunteers to assess how short-term changes in energy balance and dietary carbohydrate content impact breath acetone concentrations. Participants were studied on three occasions: on each occasion, they remained fasted and in resting conditions during the first 2 h to assess basal breath acetone and blood beta-hydroxybutyrate (BOHB). During the next 6 h, they remained fasted on one occasion (F), or were fed hourly high carbohydrate (HC) or low-carbohydrate (LC) meals to induce a positive energy balance on the other two occasions. They remained in resting conditions during 4 h, then performed a 2-hour low intensity exercise (25 W) inducing a negative energy balance. In resting conditions, breath acetone and blood BOHB concentrations increased progressively compared to basal values in F, but decreased and remained low throughout the test in HC. With LC, breath acetone increased progressively, while blood BOHB decreased. This exploratory study indicates that breath acetone reliably detects a stimulation of ketogenesis during a short-term fast. It also suggests that LC and HC differentially impact BOHB and acetone production and utilization, and reveals possible limitations to the use of breath acetone as a marker of energy balance.
Topics: 3-Hydroxybutyric Acid; Acetone; Adult; Biomarkers; Breath Tests; Energy Metabolism; Exhalation; Female; Healthy Volunteers; Humans; Male; Young Adult
PubMed: 30201981
DOI: 10.1038/s41387-018-0058-5 -
PloS One 2022Most research aimed at measuring biomarkers on the skin is only concerned with sensing chemicals in sweat using electrical signals, but these methods are not truly...
Most research aimed at measuring biomarkers on the skin is only concerned with sensing chemicals in sweat using electrical signals, but these methods are not truly non-invasive nor non-intrusive because they require substantial amounts of sweat to get a reading. This project aims to create a truly non-invasive wearable sensor that continuously detects the gaseous acetone (a biomarker related to metabolic disorders) that ambiently comes out of the skin. Composite films of polyaniline and cellulose acetate, exhibiting chemo-mechanical actuation upon exposure to gaseous acetone, were tested in the headspaces above multiple solutions containing acetone, ethanol, and water to gauge response sensitivity, selectivity, and repeatability. The bending of the films in response to exposures to these environments was tracked by an automatic video processing code, which was found to out-perform an off-the-shelf deep neural network-based tracker. Using principal component analysis, we showed that the film bending is low dimensional with over 90% of the shape changes being captured with just two parameters. We constructed forward models to predict shape changes from the known exposure history and found that a linear model can explain 40% of the observed variance in film tip angle changes. We constructed inverse models, going from third order fits of shape changes to acetone concentrations where about 45% of the acetone variation and about 30% of ethanol variation are captured by linear models, and non-linear models did not perform substantially better. This suggests there is sufficient sensitivity and inherent selectivity of the films. These models, however, provide evidence for substantial hysteretic or long-time-scale responses of the PANI films, seemingly due to the presence of water. Further experiments will allow more accurate discrimination of unknown exposure environments. Nevertheless, the sensor will operate with high selectivity in low sweat body locations, like behind the ear or on the nails.
Topics: Acetone; Ethanol; Gases; Motion Pictures; Water
PubMed: 35476814
DOI: 10.1371/journal.pone.0267311 -
International Journal of Biological... Jan 2018Technical lignins are structurally heterogeneous and polydisperse. This work describes the use of a simple and green method for lignin fractionation, using different...
Technical lignins are structurally heterogeneous and polydisperse. This work describes the use of a simple and green method for lignin fractionation, using different proportions of acetone (40 and 60%) in water. Lignins from three different sources (wheat straw organosolv lignin, wheat straw soda lignin and softwood kraft lignin) were used in this fractionation protocol. The obtained fractions showed different molar mass and functional groups. The lower molar mass fractions showed more phenolic hydroxyl groups and carboxylic acid moieties than higher molar mass fractions, which also possessed much higher amounts of carbohydrates. The chemical characterization of these fractionated lignins showed that the PREC fraction was exceptionally pure and homogeneous lignin. Its total lignin content was >96% for all three lignins and it was practically free from carbohydrates and inorganics (ash). Furthermore, PREC fraction possessed the highest carbon content for the three lignin samples (63.05-69.26%). These results illustrate that the proposed aqueous acetone fractionation protocol could indeed produce pure and uniform lignin fraction and it was applicable for lignins from different sources.
Topics: Acetone; Chemical Fractionation; Lignin; Molecular Weight; Solvents; Triticum; Water
PubMed: 28834702
DOI: 10.1016/j.ijbiomac.2017.08.102 -
Bioelectrochemistry (Amsterdam,... Aug 2023This manuscript describes the effect of altering the extracellular redox potential during the production of acetone, butanol, and ethanol on a dual chamber H-type...
This manuscript describes the effect of altering the extracellular redox potential during the production of acetone, butanol, and ethanol on a dual chamber H-type microbial fuel cell by fermenting glucose with Clostridium saccharoperbutylacetonicum N1-4. Extracellular redox potential modification was achieved by either supplementing the microbial broth with the redox agent NADH or by poising the cathode potential at -600 mV vs. Ag/AgCl. The addition of NADH was found to foment the production of acetone via fermentation of glucose. The addition of 200 mM of NADH to the catholyte rendered the highest production of acetone (2.4 g L), thus outperforming the production of acetone by conventional fermentation means (control treatment) by a factor of 2.2. The experimental evidence gathered here, indicates that cathodic electro-fermentation of glucose favors the production of butanol. When poising the cathode potential at -600 mV vs Ag/AgCl (electro-fermentation), the largest production of butanol was achieved (5.8 g L), outperforming the control treatment by a factor of 1.5. The production of ABE solvents and the electrochemical measurements demonstrate the electroactive properties of C. saccharoperbutylacetonicum N1-4 and illustrates the usefulness of bio-electrochemical systems to improve conventional fermentative processes.
Topics: Butanols; Acetone; Ethanol; Fermentation; NAD; 1-Butanol; Clostridium; Glucose
PubMed: 36940584
DOI: 10.1016/j.bioelechem.2023.108414