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European Journal of Biochemistry Aug 1969
Topics: 17-Hydroxycorticosteroids; Androstanes; Androsterone; Bile; Chemical Phenomena; Chemistry; Chromatography; Chromatography, Gas; Chromatography, Gel; Dehydroepiandrosterone; Dextrans; Etiocholanolone; Glucuronates; Glucuronidase; Humans; Methods; Pregnanediol; Pregnanes; Pregnenolone; Spectrum Analysis; Steroids; Tritium
PubMed: 4242108
DOI: 10.1111/j.1432-1033.1969.tb00669.x -
FEBS Letters Feb 1974
Comparative Study
Topics: Amino Acid Sequence; Amino Acids; Animals; Cattle; Chromatography, Ion Exchange; Collagen; Cyanogen Bromide; Electrophoresis, Disc; Humans; Peptide Fragments; Protein Denaturation; Rats; Skin; Solubility; Species Specificity; Swine; Ultracentrifugation
PubMed: 4852598
DOI: 10.1016/0014-5793(74)80047-5 -
The Journal of Biological Chemistry May 1973
Topics: Alkylation; Amino Acids; Animals; Blood Proteins; Carbohydrates; Cattle; Chromatography, DEAE-Cellulose; Chromatography, Gel; Citrates; Cysteine; Dialysis; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Factor X; Molecular Weight; Oxidation-Reduction; Peptides; Prothrombin; Sodium; Sodium Dodecyl Sulfate; Spectrophotometry; Thrombin; Trypsin
PubMed: 4702880
DOI: No ID Found -
The Journal of Biological Chemistry Oct 1968
Topics: Amino Acids; Chemical Phenomena; Chemistry; Chromatography, Ion Exchange; Chromatography, Paper; Electrophoresis; Endopeptidases; Peptides; Protein Hydrolysates; Trypsin
PubMed: 5702048
DOI: No ID Found -
The Biochemical Journal Dec 19711. The total yield of fatty acids from the whole envelopes was markedly higher than that obtained from the ordinary cell walls. In both samples the major fatty acids...
1. The total yield of fatty acids from the whole envelopes was markedly higher than that obtained from the ordinary cell walls. In both samples the major fatty acids were C(16) and C(18) acids. 2. The whole envelopes contained C(18) acids and long-chain (C(19)-C(26)) fatty acids, in a higher proportion than did the ordinary cell walls. Fifteen fatty acids with more than 18 carbon atoms were identified, among which 2-hydroxy-C(26:0) and C(26:0) acids predominated. 3. A complex sphingolipid containing inositol, phosphorus and mannose was isolated from the whole cell envelopes. The main fatty acids of this lipid were 2-hydroxy-C(26:0) and C(26:0) acids. It was concluded that this sphingolipid is present both in the ordinary cell wall and in the plasma membrane of baker's yeast. 4. The neutral lipids amounted to over 50% and the glycerophosphatides to about 30% of the total fatty acid content of the whole envelope. The major fatty acids in these lipids were C(16:1), C(18:1) and C(16:0) acids. The proportion of fatty acids with more than 18 carbon atoms was lowest in the neutral lipids, whereas the neutral glycolipids contained the highest percentage of these fatty acids. Acidic glycolipids amounted to 14% of the total fatty acid content of the whole envelope. The presence of a cerebroside sulphate in this lipid fraction was demonstrated, whereas the high content of 2-hydroxy-C(26:0) acid found is caused by the complex inositol- and mannose-containing sphingolipid.
Topics: Cell Fractionation; Cell Membrane; Cell Wall; Cerebrosides; Chromatography, Gas; Fatty Acids; Glycolipids; Inositol; Lipids; Mannose; Phospholipids; Saccharomyces; Saccharomyces cerevisiae; Sphingolipids
PubMed: 4947296
DOI: 10.1042/bj1250963 -
European Journal of Biochemistry Jul 1979A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation...
A major acidic glycolipid of the rabbit alveolar lavage has been isolated and characterized. The isolation procedure involved extraction of lipids, column fractionation on DEAE-Sephadex and silicic acid, and thin-layer chromatography. Chemical analysis of the glycolipid revealed the presence of glucose, fatty acids, glycerol mono-ethers and sulfate. By partial acid and alkaline methanolysis, oxidation with periodate and chromium trioxide, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: SO3H-6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 6Glc alpha 1 leads to 3-1,(3)-O-alkyl-2-0-acylglycerol.
Topics: Chromatography, Thin Layer; Ethers; Fatty Acids; Glucose; Glycerol; Glycolipids; Pulmonary Alveoli; Sulfuric Acids
PubMed: 467447
DOI: 10.1111/j.1432-1033.1979.tb13158.x -
The Journal of Biological Chemistry Oct 1969
Topics: Antigens; Blood Group Antigens; Chemical Phenomena; Chemistry; Chromatography, Gas; Chromatography, Paper; Erythritol; Female; Fucose; Galactose; Glucose; Hemagglutination Inhibition Tests; Humans; Hydrogen-Ion Concentration; Immune Sera; Methods; Milk, Human; Models, Structural; Oligosaccharides; X-Ray Diffraction
PubMed: 5348597
DOI: No ID Found -
Journal of Lipid Research Jul 1971Simple thin-layer chromatographic procedures are outlined for the separation, isolation, and characterization of a complex of lipophilic naphthoquinones. Procedures are... (Comparative Study)
Comparative Study
Simple thin-layer chromatographic procedures are outlined for the separation, isolation, and characterization of a complex of lipophilic naphthoquinones. Procedures are also described for the quantitative recovery of naphthoquinones from thin-layer plates. The general usefulness of the described methods is demonstrated by their application in the analysis of menaquinones from several microorganisms. The methods allow distinction between menaquinones varying in side-chain length, degree of saturation, and geometry, as well as in the presence or absence of ring methyl groups.
Topics: Bacillus; Chromatography, Thin Layer; Corynebacterium; Evaluation Studies as Topic; Flavobacterium; Hydrogen-Ion Concentration; Light; Methods; Micrococcus; Mycobacterium; Mycobacterium tuberculosis; Silver; Species Specificity; Spectrophotometry, Ultraviolet; Streptomyces; Vitamin K
PubMed: 5005959
DOI: No ID Found -
European Journal of Biochemistry Sep 1972
Topics: Amino Acids; Bacterial Proteins; Carboxypeptidases; Chemical Phenomena; Chemical Precipitation; Chemistry; Electrophoresis, Disc; Hemagglutination; Hexosamines; Humans; Immunodiffusion; Immunoglobulin G; Lysostaphin; Molecular Weight; Peptidoglycan; Spectrophotometry, Ultraviolet; Staphylococcus
PubMed: 4628411
DOI: 10.1111/j.1432-1033.1972.tb02023.x -
European Journal of Biochemistry Apr 1972
Topics: Amino Acids; Ceruloplasmin; Chromatography, Gel; Electrophoresis; Electrophoresis, Starch Gel; Glycoproteins; Guanidines; Humans; Macromolecular Substances; Molecular Weight; Peptide Hydrolases; Peptides; Protein Conformation; Ultracentrifugation; Viscosity
PubMed: 5036973
DOI: 10.1111/j.1432-1033.1972.tb01777.x