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Biochemistry and Molecular Biology... Jan 2023An undergraduate online course on Applied Biochemistry and Molecular Biology was developed through different formats of case study that included lecture, class...
An undergraduate online course on Applied Biochemistry and Molecular Biology was developed through different formats of case study that included lecture, class discussion, small-groups discussion, and individual work. Cases covering health, biotechnology, agriculture, and other issues were developed or adapted from the literature to reach the desired learning goals. Multiple web resources were employed for information integration that were presented and discussed in the synchronous sessions and assignments. Formative and summative assessment was achieved through multiple-choice questionnaires, exams, and homework assignments.
Topics: Humans; Learning; Students; Biochemistry; Curriculum; Molecular Biology
PubMed: 36169183
DOI: 10.1002/bmb.21679 -
The Journal of Biological Chemistry Jul 2020The National Science Foundation estimates that 80% of the jobs available during the next decade will require math and science skills, dictating that programs in...
The National Science Foundation estimates that 80% of the jobs available during the next decade will require math and science skills, dictating that programs in biochemistry and molecular biology must be transformative and use new pedagogical approaches and experiential learning for careers in industry, research, education, engineering, health-care professions, and other interdisciplinary fields. These efforts require an environment that values the individual student and integrates recent advances from the primary literature in the discipline, experimentally directed research, data collection and analysis, and scientific writing. Current trends shaping these efforts must include critical thinking, experimental testing, computational modeling, and inferential logic. In essence, modern biochemistry and molecular biology education must be informed by, and integrated with, cutting-edge research. This environment relies on sustained research support, commitment to providing the requisite mentoring, access to instrumentation, and state-of-the-art facilities. The academic environment must establish a culture of excellence and faculty engagement, leading to innovation in the classroom and laboratory. These efforts must not lose sight of the importance of multidimensional programs that enrich science literacy in all facets of the population, students and teachers in K-12 schools, nonbiochemistry and molecular biology students, and other stakeholders. As biochemistry and molecular biology educators, we have an obligation to provide students with the skills that allow them to be innovative and self-reliant. The next generation of biochemistry and molecular biology students must be taught proficiencies in scientific and technological literacy, the importance of the scientific discourse, and skills required for problem solvers of the 21st century.
Topics: Biochemistry; Biomedical Research; Humans; Molecular Biology; Problem-Based Learning
PubMed: 32527726
DOI: 10.1074/jbc.AW120.011104 -
IUBMB Life Apr 2001Flow cytometry (FCM) allows the simultaneous measurement of multiple fluorescences and light scatter induced by illumination of single cells or microscopic particles in... (Review)
Review
Flow cytometry (FCM) allows the simultaneous measurement of multiple fluorescences and light scatter induced by illumination of single cells or microscopic particles in suspension, as they flow rapidly through a sensing area. In some systems, individual cells or particles may be sorted according to the properties exhibited. By using appropriate fluorescent markers, FCM is unique in that multiple structural and functional parameters can be quantified simultaneously on a single-particle basis, whereas up to thousands of biological particles per second may be examined. FCM is increasingly used for basic, clinical, biotechnological, and environmental studies of biochemical relevance. In this critical review, we summarize the main advantages and limitations of FCM for biochemical studies and discuss briefly the most relevant parameters and analytical strategies. Graphical examples of the biological information provided by multiparametric FCM are presented. Also, this review contains specific sections on flow cytoenzymology, FCM analysis of isolated subcellular organelles, and cell-free FCM.
Topics: Animals; Biochemistry; Cell Separation; Flow Cytometry; Fluorescent Dyes; Humans
PubMed: 11569917
DOI: 10.1080/152165401753311771 -
Biochemistry and Molecular Biology... Nov 2020The interdisciplinary subject of agricultural biochemistry can provide an abundance of didactic opportunities for educators teaching biochemistry and molecular biology...
The interdisciplinary subject of agricultural biochemistry can provide an abundance of didactic opportunities for educators teaching biochemistry and molecular biology in secondary-level science courses. This is especially true in present times when virtual-learning strategies supersede in-person instruction and contemporary approaches are needed to engage students with relevant applications of science. In this communication, we discuss how pairing daily lessons in agricultural biochemistry with periodic e-notebook usage further refines this strategy by promoting content-retrieval and providing educators with formative feedback on student progress in a simple and inexpensive manner.
Topics: Agriculture; Biochemistry; Humans; Teaching
PubMed: 33186489
DOI: 10.1002/bmb.21467 -
BMB Reports Nov 2011Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large... (Review)
Review
Advances in the fields of proteomics and genomics have necessitated the development of high-throughput screening methods (HTS) for the systematic transformation of large amounts of biological chemical data into an organized database of knowledge. Microfluidic systems are ideally suited for high-throughput biochemical experimentation since they offer high analytical throughput, consume minute quantities of expensive biological reagents, exhibit superior sensitivity and functionality compared to traditional micro-array techniques and can be integrated within complex experimental work flows. A range of basic biochemical and molecular biological operations have been transferred to chip-based microfluidic formats over the last decade, including gene sequencing, emulsion PCR, immunoassays, electrophoresis, cell-based assays, expression cloning and macromolecule blotting. In this review, we highlight some of the recent advances in the application of microfluidics to biochemistry and molecular biology.
Topics: Biochemistry; Humans; Microfluidic Analytical Techniques; Molecular Biology; Proteomics
PubMed: 22118535
DOI: 10.5483/BMBRep.2011.44.11.705 -
Biochemistry and Molecular Biology... Jul 2018Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely...
Determination of thermostability of enzymes is of prime importance for their successful industrial applications and, yet, the published data has often been incompletely analyzed to assess the suitability of enzymes. It is possible to determine meaningful thermostability parameters from the routinely acquired data through a straightforward method that is not only more informative but also provides a means to compare thermostability of enzymes from different sources. Here, we describe a simple, effective, and economical way to determine enzyme thermostability. In our opinion, including this method in Biochemistry and Molecular Biology curricula will encourage students to include thermostability analysis in their future work, leading to a more meaningful approach to evaluate and compare enzymes. Furthermore, as the method requires minimum specialized equipment, the analysis will be particularly suitable for labs that cannot afford expensive setup. © 2018 by The International Union of Biochemistry and Molecular Biology, 46:398-402, 2018.
Topics: Biochemistry; Enzyme Stability; Enzymes; Molecular Biology; Temperature
PubMed: 29717551
DOI: 10.1002/bmb.21127 -
Protein Science : a Publication of the... Apr 2001This personal and professional autobiography covers the 50-yr period of 1950-2000 and includes the following topics: History of the University of Washington School of...
This personal and professional autobiography covers the 50-yr period of 1950-2000 and includes the following topics: History of the University of Washington School of Medicine and its Department of Biochemistry (Mount Rainier and the University of Washington, recruiting faculty, biology, research programs); scientific editing (publication, Biochemistry, Protein Science, electronic publication); Europe revisited (Heidelberg, approaching retirement, the German Research Center, reunion in Vienna); and 50 yr of research on proteolytic enzymes (trypsin, carboxypeptidases, mast cell proteases, future developments).
Topics: Biochemistry; History, 20th Century; Peptide Hydrolases; Schools, Medical; United States; Universities; Washington
PubMed: 11274481
DOI: 10.1110/ps.40101 -
Biochemistry and Molecular Biology... Nov 2020Teaching biochemistry well is not an easy job for teachers and so is learning biochemistry well for students. Just as many students often complain, they virtually always...
Teaching biochemistry well is not an easy job for teachers and so is learning biochemistry well for students. Just as many students often complain, they virtually always fail in either physiology or biochemistry. I have been teaching biochemistry at Nanjing University for over 25 years and am very successful in my biochemistry-teaching career. The way and style that I teach biochemistry is highly praised by both my students and my fellow teachers. As an instructor, the most important thing is, I think, you have to do your best to arouse or ignite students' interest in biochemistry learning. But how? In this article, I will introduce many first-hand practical tips on how to arouse the students 'interest in studying biochemistry from giving the first lecture to last lecture, from how to combine advanced teaching methods with traditional teaching methods to how to combine classic biochemistry with latest biochemical developments, from the main classroom teaching to organization of extracurricular activities as the second classroom, and so on. These tips are proven very useful and then can be followed by other professors who are teaching biochemistry. Hopefully, they can help many professors improve their skills for teaching biochemistry.
Topics: Biochemistry; Humans; Learning; Students, Medical; Teaching
PubMed: 32585751
DOI: 10.1002/bmb.21357 -
Sheng Wu Gong Cheng Xue Bao = Chinese... Apr 2022As the content of static biochemistry is boring and traditional cramming education fails to draw the attention of students, we divided the course into small units and...
As the content of static biochemistry is boring and traditional cramming education fails to draw the attention of students, we divided the course into small units and then made full use of "micro-teaching" resources such as multimedia slides, pictures, objects, experimental demonstrations and animations of the biochemical principles. The method created a "micro-teaching" environment, which combined boring static knowledge with vivid dynamic elements, thus producing a strong sensory impact on students, highlighting the knowledge in their mind, prompting students' participation, and greatly improving the teaching effects. This article introduces the goals and development process of this teaching method, and takes "Protein Denaturation and Renaturation" as an example to demonstrate the design and implementation process of the "micro-teaching", which seems to be of great significance to the improvement of the teaching quality of biochemistry course and serves as a reference for reform in other courses.
Topics: Biochemistry; Humans; Students
PubMed: 35470635
DOI: 10.13345/j.cjb.210810 -
Protein Science : a Publication of the... Mar 2017
Topics: Biochemistry; Germany; History, 20th Century; History, 21st Century; Humans; Protein Aggregates; Protein Folding; Protein Stability; Proteins
PubMed: 27977893
DOI: 10.1002/pro.3098