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Current Issues in Molecular Biology Oct 2003Polypeptide libraries cast a broad net for defining enzyme and binding protein specificities. In addition to uncovering rules for molecular recognition, the binding... (Review)
Review
Polypeptide libraries cast a broad net for defining enzyme and binding protein specificities. In addition to uncovering rules for molecular recognition, the binding preferences and functional group tolerances from such libraries can reveal mechanisms underlying biochemical and cellular processes. Ligands obtained from protein libraries can also provide pharmaceutical lead compounds and even reagents to further explore cell biology. Here, we review selected recent examples of protein libraries demonstrating these principles. In particular, we focus on combinatorial libraries composed of randomized peptides or variations of a single protein. The characteristics of various techniques for library constructions and screening are also briefly surveyed.
Topics: Animals; Biochemistry; Cell Physiological Phenomena; Humans; Molecular Biology; Peptide Library; Proteins
PubMed: 12921228
DOI: No ID Found -
FEBS Open Bio Sep 2020When developing meaningful curricula, institutions must engage with the desired disciplinary attributes of their graduates. Successfully employed in several areas,...
When developing meaningful curricula, institutions must engage with the desired disciplinary attributes of their graduates. Successfully employed in several areas, including psychology and chemistry, disciplinary literacies provide structure for the development of core competencies-pursuing progressive education. To this end, we have sought to develop a comprehensive blueprint of a graduate biochemist, providing detailed insight into the development of skills in the context of disciplinary knowledge. The Biochemical Literacy Framework (BCLF) aspires to encourage innovative course design in both the biochemical field and beyond through stimulating discussion among individuals developing undergraduate biochemistry degree courses based on pedagogical best practice. Here, we examine the concept of biochemical literacy aiming to start answering the question: What must individuals do and know to approach and transform ideas in the context of the biochemical sciences? The BCLF began with the guidance published by relevant learned societies - including the Royal Society of Biology, the Biochemical Society, the American Society for Biochemistry and Molecular Biology and the Quality Assurance Agency, before considering relevant pedagogical literature. We propose that biochemical literacy is comprised of seven key skills: critical thinking, self-management, communication, information literacy, visual literacy, practical skills and content knowledge. Together, these form a dynamic, highly interconnected and interrelated meta-literacy supporting the use of evidence-based, robust learning techniques. The BCLF is intended to form the foundation for discussion between colleagues, in addition to forming the groundwork for both pragmatic and exploratory future studies into facilitating and further defining biochemical literacy.
Topics: Biochemistry; Curriculum; Educational Measurement; Humans; Learning; Literacy; Molecular Biology; Students
PubMed: 32696491
DOI: 10.1002/2211-5463.12938 -
Biochemistry and Molecular Biology... May 2022Laboratory-based practical classes are an essential component in teaching molecular biology for undergraduate students. Universidad Regional Amazonica Ikiam is a higher...
Laboratory-based practical classes are an essential component in teaching molecular biology for undergraduate students. Universidad Regional Amazonica Ikiam is a higher education institution located in the Ecuadorian Amazon rainforest, a high biodiversity place, including amphibians. Based on this, we have established a practical molecular biology program with eight sessions that contextualize the biodiverse surroundings of the University. This program stimulates synchronization of information between theory and practice and improves research skills. During these sessions, students are motivated to identify and characterize antimicrobial peptides from Ecuadorian frog skin secretions, using molecular biology techniques and biochemistry and microbiology knowledge. This practical course was held twice with a total of 56 students from the fifth semester of the biotechnology engineering. The evaluation of the practical program was carried out through a questionnaire applied to students using the Likert scale. Overall, this form of teaching had high receptivity and presented benefits for student learning. Interestingly, 80% of respondents strongly agreed that this course provided tools and knowledge for the development of their undergraduate dissertation. Therefore, practical courses tailored to the student's context can stimulate student learning and interest. Additionally, this experimental methodology is interdisciplinary and can be applied to other research fields and subjects.
Topics: Biochemistry; Curriculum; Humans; Molecular Biology; Students; Universities
PubMed: 35263036
DOI: 10.1002/bmb.21612 -
Biochemistry Jan 2018O-Linked β-N-acetylglucosamine (O-GlcNAc) is a critical post-translational modification (PTM) of thousands of intracellular proteins. Reversible O-GlcNAcylation governs... (Review)
Review
O-Linked β-N-acetylglucosamine (O-GlcNAc) is a critical post-translational modification (PTM) of thousands of intracellular proteins. Reversible O-GlcNAcylation governs many aspects of cell physiology and is dysregulated in numerous human diseases. Despite this broad pathophysiological significance, major aspects of O-GlcNAc signaling remain poorly understood, including the biochemical mechanisms through which O-GlcNAc transduces information. Recent work from many laboratories, including our own, has revealed that O-GlcNAc, like other intracellular PTMs, can control its substrates' functions by inhibiting or inducing protein-protein interactions. This dynamic regulation of multiprotein complexes exerts diverse downstream signaling effects in a range of processes, cell types, and organisms. Here, we review the literature about O-GlcNAc-regulated protein-protein interactions and suggest important questions for future studies in the field.
Topics: Acetylglucosamine; Aminoacylation; Animals; Biochemistry; Humans; Models, Biological; Protein Interaction Domains and Motifs; Protein Multimerization; Protein Processing, Post-Translational; Signal Transduction
PubMed: 29099585
DOI: 10.1021/acs.biochem.7b00871 -
British Medical Journal Sep 1973
Topics: Biochemistry; History, 20th Century; Russia (Pre-1917)
PubMed: 4580027
DOI: No ID Found -
The Journal of Antibiotics Jul 2002
Topics: Biochemistry; Isomerism; Lactones
PubMed: 12243458
DOI: 10.7164/antibiotics.55.663 -
Science (New York, N.Y.) Apr 1999"Complexity" is a subject that is beginning to be important in chemistry. Historically, chemistry has emphasized the approximation of complex nonlinear processes by...
"Complexity" is a subject that is beginning to be important in chemistry. Historically, chemistry has emphasized the approximation of complex nonlinear processes by simpler linear ones. Complexity is becoming a profitable approach to a wide range of problems, especially the understanding of life.
Topics: Biochemistry; Chemistry; Models, Chemical; Molecular Conformation; Neural Networks, Computer; Thermodynamics
PubMed: 10102824
DOI: 10.1126/science.284.5411.89 -
The Journal of Biological Chemistry Feb 2004
Topics: Allergy and Immunology; Biochemistry; Brain; Cells; Epitopes; History, 20th Century; History, 21st Century; Homeostasis; Molecular Biology; Morphogenesis; Selection, Genetic
PubMed: 14718522
DOI: 10.1074/jbc.X400001200 -
CBE Life Sciences Education Dec 2018
Topics: Accreditation; Biochemistry; Biological Science Disciplines; Humans; Molecular Biology
PubMed: 30444448
DOI: 10.1187/cbe.18-09-0189 -
Biochemistry and Molecular Biology... 2016Molecular dynamics (MD) simulations play an increasingly important role in many aspects of biochemical research but are often not part of the biochemistry curricula at...
Molecular dynamics (MD) simulations play an increasingly important role in many aspects of biochemical research but are often not part of the biochemistry curricula at the undergraduate level. This article discusses the pedagogical value of exposing students to MD simulations and provides information to help instructors consider what software and hardware resources are necessary to successfully introduce these simulations into their courses. In addition, a brief review of the MD-based activities in this issue and other sources are provided.
Topics: Biochemistry; Molecular Dynamics Simulation; Software; Students; Teaching
PubMed: 27001155
DOI: 10.1002/bmb.20943