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Turkish Journal of Surgery Dec 2019In the present study, it was aimed to study the antioxidant effects of spironolactone (SPL) to determine its possible protective effects in hepatic ischemia reperfusion...
OBJECTIVES
In the present study, it was aimed to study the antioxidant effects of spironolactone (SPL) to determine its possible protective effects in hepatic ischemia reperfusion injury.
MATERIAL AND METHODS
Hepatic artery, portal vein, and bile duct of Wistar albino rats were clamped for 45 minutes under anesthesia to form an ischemia period. Then reperfusion was allowed and the rats were decapitated 60 minutes later. SPL (20 mg/kg, p.o.) or SF was orally administered for 30 minutes before ischemia. Rats in the control arm underwent sham surgery and were administered isotonic saline. Liver function was studied by measuring aspartate aminotransferase (AST), alanine aminotransferase (ALT), tumor necrosis factor-alpha (TNF-α), and interleukin 1beta (IL-1β) levels. Malondialdehyde (MDA), glutathione (GSH), luminol, and lucigenin levels, myeloperoxidase (MPO) and Na+-K+-ATPase enzyme activities were analyzed to study tissue injury under light microscope.
RESULTS
While IR increased AST, ALT, TNF-α, and IL-1β levels and MDA, luminol, and lusigenin levels and MPO activities, it caused a decrease in GSH levels and Na+K+-ATPase activity. Spironolactone administration significantly improved these values.
CONCLUSION
Protective effects of SPL against ischemia/reperfusion injury via various mechanisms suggest that this agent may become a novel treatment agent in clinical practice.
PubMed: 32551425
DOI: 10.5578/turkjsurg.4340 -
Pakistan Journal of Medical Sciences 2017To compare the effect of exercise and morphine on abstinence syndrome and hippocampal gene expression in rat model.
OBJECTIVES
To compare the effect of exercise and morphine on abstinence syndrome and hippocampal gene expression in rat model.
METHODS
Thirty adult male rats were exposed to voluntary wheel exercise (low, medium, high) for 28 days. The subjects entered Conditioned Place Preference (CPP) apparatus and experienced morphine (low, medium, high) CPP and followed by naloxone test. Correlation between exercise level, morphine injection, concurrent morphine administration and exercise with morphine CPP, BDNF and TrkB genes was determined. Rats were euthanized, decapitated and the hippocampus was removed. The expression of BDNF and TrkB genes were evaluated by real time PCR.
RESULTS
Active rats ran an average of 839.18 m/d. A significant (P<0.001) correlation between exercise level, morphine injection, concurrent morphine administration and exercise with morphine CPP and BDNFand TrKB gene expressions was found.
CONCLUSION
Voluntary exercise in different levels potentiates the brain rewarding system, CPP scale, and hippocampal BDNF and TrKB expressions. High range of voluntary exercise demonstrated an increase in the likelihood of developing addictive and drug-seeking behavior.
PubMed: 28811779
DOI: 10.12669/pjms.333.12342 -
BMC Plant Biology Apr 2023Sunflower is an important ornamental plant, which can be used for fresh cut flowers and potted plants. Plant architecture regulation is an important agronomic operation...
BACKGROUND
Sunflower is an important ornamental plant, which can be used for fresh cut flowers and potted plants. Plant architecture regulation is an important agronomic operation in its cultivation and production. As an important aspect of plant architecture formation, shoot branching has become an important research direction of sunflower.
RESULTS
TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors are essential in regulating various development process. However, the role of TCPs in sunflowers has not yet been studied. This study, 34 HaTCP genes were identified and classified into three subfamilies based on the conservative domain and phylogenetic analysis. Most of the HaTCPs in the same subfamily displayed similar gene and motif structures. Promoter sequence analysis has demonstrated the presence of multiple stress and hormone-related cis-elements in the HaTCP family. Expression patterns of HaTCPs revealed several HaTCP genes expressed highest in buds and could respond to decapitation. Subcellular localization analysis showed that HaTCP1 was located in the nucleus. Paclobutrazol (PAC) and 1-naphthylphthalamic acid (NPA) administration significantly delayed the formation of axillary buds after decapitation, and this suppression was partially accomplished by enhancing the expression of HaTCP1. Furthermore, HaTCP1 overexpressed in Arabidopsis caused a significant decrease in branch number, indicating that HaTCP1 played a key role in negatively regulating sunflower branching.
CONCLUSIONS
This study not only provided the systematic analysis for the HaTCP members, including classification, conserved domain and gene structure, expansion pattern of different tissues or after decapitation. But also studied the expression, subcellular localization and function of HaTCP1. These findings could lay a critical foundation for further exploring the functions of HaTCPs.
Topics: Transcription Factors; Helianthus; Plant Proteins; Phylogeny; Decapitation; Gene Expression Regulation, Plant; Arabidopsis
PubMed: 37101166
DOI: 10.1186/s12870-023-04211-0 -
Biology May 2021Decapitation is common in horticulture for altering plant architecture. The decapitation of chrysanthemum plants breaks apical dominance and leads to more flowers on...
Decapitation is common in horticulture for altering plant architecture. The decapitation of chrysanthemum plants breaks apical dominance and leads to more flowers on lateral branches, resulting in landscape flowers with good coverage. We performed both third- and second-generation transcriptome sequencing of the second buds of chrysanthemum. This third-generation transcriptome is the first sequenced third-generation transcriptome of chrysanthemum, revealing alternative splicing events, lncRNAs, and transcription factors. Aside from the classic hormones, the expression of jasmonate-related genes changed because of this process. Sugars also played an important role in this process, with upregulated expression of sucrose transport-related and genes. We constructed a model of the initial growth of the second buds after decapitation. Auxin export and sugar influx activated the growth of these buds, while the JA-Ile caused by wounding inhibited the expression of genes from 0 h to 6 h. After wound recovery, cytokinins accumulated in the second buds and might have induced expression to upregulate gene expression from 6 h to 48 h, together with sugars. Therefore, jasmonates, cytokinins, sugars, and auxin work together to determine the fate of the buds of plants with short internodes, such as chrysanthemum.
PubMed: 34065759
DOI: 10.3390/biology10050440 -
Turkish Neurosurgery Dec 2021Peripheral nerve regeneration remains an issue, and novel therapeutic approaches are required for functional recovery. This study investigated the regenerative potential...
AIM
Peripheral nerve regeneration remains an issue, and novel therapeutic approaches are required for functional recovery. This study investigated the regenerative potential and long-term functional effects of Uridine treatment in a rat model of sciatic nerve injury.
MATERIAL AND METHODS
Male Sprague-Dawley rats were randomized to receive sham surgery plus saline (Sham group), right sciatic nerve transection and primary repair plus saline (Control group), right sciatic nerve transection, and primary repair plus 500 mg/kg Uridine (Uridine group). Saline or Uridine was injected intraperitoneally (i.p.) for seven days, and the rats were monitored for 12 weeks after surgery. We evaluated electrophysiological and functional recovery using electromyography (EMG) and sciatic functional index (SFI) at six and 12 weeks, respectively. At 12 weeks, rats were decapitated and their right sciatic nerves were examined in macroscopic and histomorphologic manners.
RESULTS
Functional evaluation by SFI and sciatic nerve conduction velocity analyzed by EMG both decreased in the Control group but recovered in the Uridine group 12 weeks after surgery. Additionally, upon experiment completion, Uridine treatment was observed to enhance nerve adherence, separability scores, and the number of myelinated axons.
CONCLUSION
These results reveal that short-term Uridine treatment provides morphological and electrophysiological benefits, which are represented by long-term functional improvement in a rat model of sciatic nerve injury. These findings validate and extend our knowledge on Uridine's regenerative effects in peripheral nerve injuries.
PubMed: 35652178
DOI: 10.5137/1019-5149.JTN.36142-21.2 -
La Clinica Terapeutica 2023Each year, there are billions of agricultural work accidents involving the operation of tractors, grain augers, harvest combines, power take-off devices, or balers and...
BACKGROUND
Each year, there are billions of agricultural work accidents involving the operation of tractors, grain augers, harvest combines, power take-off devices, or balers and thrashers. Field accidents of this nature seem more common on afternoons, just as road accidents tend to skew toward nighttime. The lesions can vary widely and depends strictly on the operation of the machinery analyzed.
AIMS
This paper aims to present a peculiar case of decapitation by a combine harvester, showing how, in cases of injury due to agricultural machinery, it is fundamental a correct execution of a scene investigation, autoptic examination, and cooperation with a specialist in engineering.
CASE REPORT
A 54-year-old man was found decapitated on the header of a combine harvester; his extremities were also dismembered. At autopsy, a clean oblique cut across the first cervical vertebra had severed the head at the neck. Although the right arm remained intact, both lower extremities were mutilated, showing numerous exposed and open fractures. A bleeding, penetrating wound to the back was additionally noted. In the days that followed, missing parts (head and left leg) were discovered in other machine components (grain tank and straw walker, respectively). All observed injuries were compatible with the mechanics of the cochlea, its rotating movement inflicting the damages above. Collaboration between pathologists and engineers was fundamental to recreating the dynamics of this rare decapitation accident by a combine harvester.
Topics: Male; Humans; Middle Aged; Decapitation; Farmers; Neck; Autopsy
PubMed: 37674446
DOI: 10.7417/CT.2023.2454 -
Insects Feb 2020flies (Diptera: Phoridae) parasitize individual ant workers, causing decapitation of the host during pupariation. Phorid flies that attack South American fire ants in... (Review)
Review
flies (Diptera: Phoridae) parasitize individual ant workers, causing decapitation of the host during pupariation. Phorid flies that attack South American fire ants in the (Smith) complex are distributed across a wide range of habitats and climates associated with the geographical range of their hosts. Sympatric species sharing the same hosts often partition niche resources by season, active time of day, host size, and/or different host activities. They have the potential of being used for biological control of the imported fire ants in North America, Australia, and Asia.
PubMed: 32041256
DOI: 10.3390/insects11020107 -
Biology Open Nov 2015The ability of some animals to regrow their head and brain after decapitation provides a striking example of the regenerative capacity within the animal kingdom. The...
The ability of some animals to regrow their head and brain after decapitation provides a striking example of the regenerative capacity within the animal kingdom. The acoel worm Symsagittifera roscoffensis can regrow its head, brain and sensory head organs within only a few weeks after decapitation. How rapidly and to what degree it also reacquires its functionality to control behavior however remains unknown. We provide here a neuroanatomical map of the brain neuropils of the adult S. roscoffensis and show that after decapitation a normal neuroanatomical organization of the brain is restored in the majority of animals. By testing different behaviors we further show that functionality of both sensory perception and the underlying brain architecture are restored within weeks after decapitation. Interestingly not all behaviors are restored at the same speed and to the same extent. While we find that phototaxis recovered rapidly, geotaxis is not restored within 7 weeks. Our findings show that regeneration of the head, sensory organs and brain result in the restoration of directed navigation behavior, suggesting a tight coordination in the regeneration of certain sensory organs with that of their underlying neural circuits. Thus, at least in S. roscoffensis, the regenerative capacity of different sensory modalities follows distinct paths.
PubMed: 26581588
DOI: 10.1242/bio.014266 -
Metabolism Open Dec 2020is a folklore plant in Africa, particularly Nigeria, where its consumption is believed to stimulate red blood cells production. On this basis, the erythropoietic...
is a folklore plant in Africa, particularly Nigeria, where its consumption is believed to stimulate red blood cells production. On this basis, the erythropoietic potential of the plant was evaluated in Cephalosporin-induced anaemia model, using Wistar rats as experimental subjects. Thirty-two male rats were randomly assigned to four groups (n = 8). Group 1 animals served as control, and experimental anaemia was induced in other groups of animals via oral administration of cephalosporin (10 mg/kg BW) for a period of seven days. Animals in groups III and IV were treated orally with aqueous extract of at respective dosage of 250 and 500 mg/kg BW, twice daily for a period of 10 days; while group II animals were left untreated. All animals were thereafter fasted overnight and sacrificed by cervical decapitation. Blood was collected via the retro-orbital sinus and used for biochemical analyses. The results obtained showed that cephalosporin effectively induced anaemia as evidenced by significant changes in erythropoietic indices of the untreated anaemic animals. Treatment of anaemic animals with . particularly at a dosage of 250 mg/kg BW significantly (P˂0.05) boosted the levels of RBC (35.8%), Hb (25.2%), PCV (39.4%), cobalt (70.9%), vitamin C (82.6%), and concomitantly decreased erythropoietin level (18%) relative to untreated anaemic animals. The observations made in this study support the local use of as blood tonic and therapy for anaemia. The botanical may therefore be a useful supplement for patients placed on antibiotics which are often associated with haemolysis.
PubMed: 33103103
DOI: 10.1016/j.metop.2020.100064 -
PloS One 2022This protocol is a practical guide for preparing acute coronal slices from the midbrain of young adult mice for electrophysiology experiments. It describes two different...
This protocol is a practical guide for preparing acute coronal slices from the midbrain of young adult mice for electrophysiology experiments. It describes two different sets of solutions with their respective incubation strategies and two alternative procedures for brain extraction: decapitation under terminal isoflurane anaesthesia and intracardial perfusion with artificial cerebrospinal fluid under terminal isoflurane anaesthesia. Slices can be prepared from wild-type mice as well as from mice that have been genetically modified or transfected with viral constructs to label subsets of cells. The preparation can be used to investigate the electrophysiological properties of midbrain neurons in combination with pharmacology, opto- and chemogenetic manipulations, and calcium imaging; which can be followed by morphological reconstruction, immunohistochemistry, or single-cell transcriptomics. The protocol also provides a detailed list of materials and reagents including the design for a low-cost and easy to assemble 3D printed slice recovery chamber, general advice for troubleshooting common issues leading to suboptimal slice quality, and some suggestions to ensure good maintenance of a patch-clamp rig.
Topics: Animals; Brain; Isoflurane; Mice; Neurons; Periaqueductal Gray; Superior Colliculi
PubMed: 35951507
DOI: 10.1371/journal.pone.0271832