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PloS One 2014To identify the seasonal pattern of nitrogen (N) and phosphorus (P) limitation of phytoplankton in four different lakes, biweekly experiments were conducted from the end...
To identify the seasonal pattern of nitrogen (N) and phosphorus (P) limitation of phytoplankton in four different lakes, biweekly experiments were conducted from the end of March to September 2011. Lake water samples were enriched with N, P or both nutrients and incubated under two different light intensities. Chlorophyll a fluorescence (Chla) was measured and a model selection procedure was used to assign bioassay outcomes to different limitation categories. N and P were both limiting at some point. For the shallow lakes there was a trend from P limitation in spring to N or light limitation later in the year, while the deep lake remained predominantly P limited. To determine the ability of in-lake N:P ratios to predict the relative strength of N vs. P limitation, three separate regression models were fit with the log-transformed ratio of Chla of the P and N treatments (Response ratio = RR) as the response variable and those of ambient total phosphorus:total nitrogen (TN:TP), dissolved inorganic nitrogen:soluble reactive phosphorus (DIN:SRP), TN:SRP and DIN:TP mass ratios as predictors. All four N:P ratios had significant positive relationships with RR, such that high N:P ratios were associated with P limitation and low N:P ratios with N limitation. The TN:TP and DIN:TP ratios performed better than the DIN:SRP and TN:SRP in terms of misclassification rate and the DIN:TP ratio had the highest R₂ value. Nitrogen limitation was predictable, frequent and persistent, suggesting that nitrogen reduction could play a role in water quality management. However, there is still uncertainty about the efficacy of N restriction to control populations of N₂ fixing cyanobacteria.
Topics: Chlorophyll; Chlorophyll A; Cyanobacteria; Germany; Lakes; Nitrogen; Phosphorus; Phytoplankton; Seasons
PubMed: 24755935
DOI: 10.1371/journal.pone.0096065 -
PloS One 2020The aim of the study was to determine whether the level and form of Cr in the diet of chickens influences its accumulation in tissues as well as intestinal absorption of...
Estimated intestinal absorption of phosphorus and its deposition in chosen tissues, bones and feathers of chickens receiving chromium picolinate or chromium nanoparticles in diet.
The aim of the study was to determine whether the level and form of Cr in the diet of chickens influences its accumulation in tissues as well as intestinal absorption of P and its deposition in tissues. The experiment was carried out on 405 one-day-old male Ross 308 chickens that were randomly divided into five treatment groups. Control group was fed the diet without supplemental chromium; experimental groups were fed the diet with 3 or 6 mg/kg chromium picolinate (Cr-Pic) and with 3 or 6 mg/kg chromium nanoparticles (Cr-NP). Chromium was found to accumulate in the tissues of the ileum, liver, breast muscle, bones skin and in feathers of chickens. Chromium deposited in the ileum of chickens does not affect the ex vivo estimated intestinal absorption of P. The use of Cr in the diet of chickens carries the risk of lowering P levels in femur.
Topics: Animals; Blood Glucose; Bone and Bones; Chickens; Feathers; Intestinal Absorption; Liver; Phosphorus; Picolinic Acids; Tissue Distribution
PubMed: 33237949
DOI: 10.1371/journal.pone.0242820 -
PloS One 2011External nutrient discharges have caused eutrophication in many estuaries and coastal seas such as the Baltic Sea. The sedimented nutrients can affect bacterial...
BACKGROUND
External nutrient discharges have caused eutrophication in many estuaries and coastal seas such as the Baltic Sea. The sedimented nutrients can affect bacterial communities which, in turn, are widely believed to contribute to release of nutrients such as phosphorus from the sediment.
METHODS
We investigated relationships between bacterial communities and chemical forms of phosphorus as well as elements involved in its cycling in brackish sediments using up-to-date multivariate statistical methods. Bacterial community composition was determined by terminal restriction fragment length polymorphism and cloning of the 16S rRNA gene.
RESULTS AND CONCLUSIONS
The bacterial community composition differed along gradients of nutrients, especially of different phosphorus forms, from the estuary receiving agricultural phosphorus loading to the open sea. This suggests that the chemical composition of sediment phosphorus, which has been affected by riverine phosphorus loading, influenced on bacterial communities. Chemical and spatial parameters explained 25% and 11% of the variation in bacterial communities. Deltaproteobacteria, presumptively sulphate and sulphur/iron reducing, were strongly associated to chemical parameters, also when spatial autocorrelation was taken into account. Sulphate reducers correlated positively with labile organic phosphorus and total nitrogen in the open sea sediments. Sulphur/iron reducers and sulphate reducers linked to iron reduction correlated positively with aluminium- and iron-bound phosphorus, and total iron in the estuary. The sulphate and sulphur/iron reducing bacteria can thus have an important role both in the mineralization and mobilization of nutrients from sediment.
SIGNIFICANCE
Novelty in our study is that relationships between bacterial community composition and different phosphorus forms, instead of total phosphorus, were investigated. Total phosphorus does not necessarily bring out interactions between bacteria and phosphorus chemistry since proportions of easily usable mobile (reactive) phosphorus and immobile phosphorus forms in different sediments can vary. Our study suggested possible feedbacks between different forms of phosphorus and bacterial community composition.
Topics: Agriculture; Bacteria; Environment; Feedback; Geologic Sediments; Oceans and Seas; Phosphorus; Sulfates
PubMed: 21747910
DOI: 10.1371/journal.pone.0021555 -
Environmental Monitoring and Assessment Jul 2023Heterogeneous flow pathways through the soil determine the transport of dissolved and particle-bound nutritional elements like phosphorus (P) to ground and surface...
Heterogeneous flow pathways through the soil determine the transport of dissolved and particle-bound nutritional elements like phosphorus (P) to ground and surface waters. This study was designed to understand the spatial patterns of P in agriculturally used soils and the mechanisms causing P accumulation and depletion at the centimetre scale. We conducted dye tracer experiments using Brilliant Blue on a loamy Stagnosol in North-Eastern-Germany. The plant-available P was analysed using double lactate extraction (DL-P). The plant-available P content of the topsoil was significantly higher than that of the subsoil in all three replicates (p < 0.001). The topsoil's stained areas showed significantly higher P contents than unstained areas (p < 0.05), while the opposite was found for the subsoil. The P content varied enormously across all observed soil profiles (4 to 112 mg P kg soil) and different categories of flow patterns (matrix flow, flow fingers, macropore flow, and no visible transport pathways). The P contents of these transport pathways differed significantly and followed the order: P > P > P > P. We conclude that P tends to accumulate along flow pathways in the topsoil in the observed fertilized and tilled mineral soil. In contrast, in the subsoil at a generally lower P level, P is depleted from the prominent macroporous flow domains.
Topics: Soil; Phosphorus; Environmental Monitoring; Minerals; Soil Pollutants
PubMed: 37436525
DOI: 10.1007/s10661-023-11465-6 -
Ambio Nov 2020Phosphorus retention and bank erosion was investigated in two types of buffer zones in cereal fields in Norway: zones used for grass production and zones with natural...
Phosphorus retention and bank erosion was investigated in two types of buffer zones in cereal fields in Norway: zones used for grass production and zones with natural vegetation. Farmers' views on the two types of buffer zones were collected through questionnaires and in-depth interviews. Our results indicate that the grassed buffer zones had higher levels of plant-available phosphorus and lower infiltration rates than the natural ones. Bank erosion was higher in zones with grass production than those with trees. Interviews with farmers revealed diverging opinions on the zones. Most farmers were sceptical to natural vegetation with trees, whereas farmers who had already planted trees in the riparian zones were generally satisfied. Buffer zones can have many different functions, and we conclude that a holistic approach is needed when assessing the usefulness of this measure, taking into account water quality, biodiversity and the production of food, fodder and biomass.
Topics: Agriculture; Farmers; Humans; Norway; Phosphorus; Poaceae; Trees
PubMed: 32930956
DOI: 10.1007/s13280-020-01361-5 -
Proceedings of the National Academy of... Dec 2015More than 70,000 large dams have been built worldwide. With growing water stress and demand for energy, this number will continue to increase in the foreseeable future....
More than 70,000 large dams have been built worldwide. With growing water stress and demand for energy, this number will continue to increase in the foreseeable future. Damming greatly modifies the ecological functioning of river systems. In particular, dam reservoirs sequester nutrient elements and, hence, reduce downstream transfer of nutrients to floodplains, lakes, wetlands, and coastal marine environments. Here, we quantify the global impact of dams on the riverine fluxes and speciation of the limiting nutrient phosphorus (P), using a mechanistic modeling approach that accounts for the in-reservoir biogeochemical transformations of P. According to the model calculations, the mass of total P (TP) trapped in reservoirs nearly doubled between 1970 and 2000, reaching 42 Gmol y(-1), or 12% of the global river TP load in 2000. Because of the current surge in dam building, we project that by 2030, about 17% of the global river TP load will be sequestered in reservoir sediments. The largest projected increases in TP and reactive P (RP) retention by damming will take place in Asia and South America, especially in the Yangtze, Mekong, and Amazon drainage basins. Despite the large P retention capacity of reservoirs, the export of RP from watersheds will continue to grow unless additional measures are taken to curb anthropogenic P emissions.
Topics: Eutrophication; Monte Carlo Method; Phosphorus; Rivers
PubMed: 26644553
DOI: 10.1073/pnas.1511797112 -
Metabolic Engineering Jul 2019Cyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater...
Cyanobacteria, such as Synechococcus sp. PCC 7002 (Syn7002), are promising chassis strains for "green" biotechnological applications as they can be grown in seawater using oxygenic photosynthesis to fix carbon dioxide into biomass. Their other major nutritional requirements for efficient growth are sources of nitrogen (N) and phosphorus (P). As these organisms are more economically cultivated in outdoor open systems, there is a need to develop cost-effective approaches to prevent the growth of contaminating organisms, especially as the use of antibiotic selection markers is neither economically feasible nor ecologically desirable due to the risk of horizontal gene transfer. Here we have introduced a synthetic melamine degradation pathway into Syn7002 and evolved the resulting strain to efficiently use the nitrogen-rich xenobiotic compound melamine as the sole N source. We also show that expression of phosphite dehydrogenase in the absence of its cognate phosphite transporter permits growth of Syn7002 on phosphite and can be used as a selectable marker in Syn7002. We combined these two strategies to generate a strain that can grow on melamine and phosphite as sole N and P sources, respectively. This strain is able to resist deliberate contamination in large excess and should be a useful chassis for metabolic engineering and biotechnological applications using cyanobacteria.
Topics: Nitrogen; Phosphorus; Synechococcus
PubMed: 31063791
DOI: 10.1016/j.ymben.2019.04.013 -
Plant Physiology Jul 2011
Review
Update on phosphorus nutrition in Proteaceae. Phosphorus nutrition of proteaceae in severely phosphorus-impoverished soils: are there lessons to be learned for future crops?
Topics: Crops, Agricultural; Phosphorus; Plant Leaves; Plant Roots; Proteaceae; Soil
PubMed: 21498583
DOI: 10.1104/pp.111.174318 -
Poultry Science Feb 2007The effects of a reduced dietary nonphytate phosphorus (NPP) level and inclusion of phytase on broiler breeder performance and P concentrations in the litter and manure... (Randomized Controlled Trial)
Randomized Controlled Trial
The effects of a reduced dietary nonphytate phosphorus (NPP) level and inclusion of phytase on broiler breeder performance and P concentrations in the litter and manure were investigated. Ross 308 broiler breeder pullets and Ross 344 cockerels were placed sex-separate in a blackout growing house and fed standard starter and grower diets to 9 wk of age. At 10 wk of age, 4 treatments (A, B, C, D) were assigned to each of 4 floor pens of 68 pullets and 1 pen of 50 cockerels. From 10 to 21 wk, treatments A to D contained 0.37, 0.27, 0.27, and 0.17% NPP, respectively, with 300 phytase units (FTU)/kg of phytase added to treatments B and D. At 21 wk of age, birds were photostimulated and transferred to a two-thirds slat-litter breeder house with 16 pens of 60 pullets and 6 cockerels. A laying diet was fed from 22 to 64 wk and NPP levels of treatments A to D were adjusted to 0.37, 0.27, 0.19, and 0.09%, respectively, and phytase addition to treatments B and D was increased to 500 FTU/kg. Analysis of the litter from growing pens showed no effect on litter total P when phytase replaced 0.1% of NPP. However, decreasing the dietary NPP by 0.1% without phytase reduced the litter total P by 18%. Water-soluble P (WSP) and the WSP:total P ratio decreased when the grower dietary NPP level was reduced to 0.17% with added phytase and was correlated with litter moisture levels in growing pens. During the laying period, a reduction in NPP from 0.37 to 0.09% with added phytase reduced both the manure total P and WSP by 42%. Hen-day egg production was highest on the lowest NPP diet with phytase, but fertility decreased when the dietary NPP was reduced below 0.37%. Results showed that phytase inclusion in a broiler breeder laying diet at the expense of all added P from dicalcium phosphate reduced the manure total P and WSP concentrations by 42%, with no effect on the number of chicks produced per hen housed.
Topics: 6-Phytase; Animal Feed; Animal Nutritional Physiological Phenomena; Animals; Biological Availability; Chickens; Diet; Dietary Supplements; Feces; Female; Male; Oviposition; Phosphorus; Weight Gain
PubMed: 17234834
DOI: 10.1093/ps/86.2.225 -
Chemistry (Weinheim An Der Bergstrasse,... Sep 2022The fine-tuning of metal-phosphine-catalyzed reactions relies largely on accessing ever more precisely tuned phosphine ligands by de-novo synthesis. Late-stage C-H...
The fine-tuning of metal-phosphine-catalyzed reactions relies largely on accessing ever more precisely tuned phosphine ligands by de-novo synthesis. Late-stage C-H functionalization and diversification of commercial phosphines offers rapid access to entire libraries of derivatives based on privileged scaffolds. But existing routes, relying on phosphorus-directed transformations, only yield functionalization of C -H bonds in a specific position relative to phosphorus. In contrast to phosphorus-directed strategies, herein we disclose an orthogonal functionalization strategy capable of introducing a range of substituents into previously inaccessible positions on arylphosphines. The strongly coordinating phosphine group acts solely as a bystander in the sterically controlled borylation of bulky phosphines, and the resulting borylated phosphines serve as the supporting ligands for palladium during diversification through phosphine self-assisted Suzuki-Miyaura reactions.
Topics: Catalysis; Ligands; Palladium; Phosphines; Phosphorus
PubMed: 35789048
DOI: 10.1002/chem.202202074