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European Journal of Biochemistry Jun 1969
Topics: Animals; Chromatography, Gas; Feces; Germ-Free Life; Pregnanediol; Pregnenolone; Rats; Spectrum Analysis; Steroids
PubMed: 5804501
DOI: 10.1111/j.1432-1033.1969.tb00607.x -
The Biochemical Journal Feb 19711. Purified ovomucin was isolated as an insoluble glycoprotein complex from thick egg white. 2. A homogeneous glycoprotein, designated alpha-ovomucin, of molecular...
1. Purified ovomucin was isolated as an insoluble glycoprotein complex from thick egg white. 2. A homogeneous glycoprotein, designated alpha-ovomucin, of molecular weight 210000 and containing N-acetylglucosamine (6.7%, w/w), N-acetylgalactosamine (0.6%, w/w), galactose (1.8%, w/w), mannose (4.6%, w/w), N-acetylneuraminic acid (1.0%, w/w) and sulphate (0.7%, w/w), was isolated from preparations of reduced ovomucin by sedimentation equilibrium in a density gradient of caesium chloride formed in the presence of 4m-guanidine hydrochloride. 3. A carbohydrate-rich fraction, designated beta-ovomucin (which is homogeneous by sedimentation-velocity analysis in 5m-guanidine hydrochloride but which is heterogeneous by analytical sedimentation equilibrium in a density gradient of caesium chloride in the presence of 4m-guanidine hydrochloride), containing N-acetylglucosamine (11.0%, w/w), N-acetylgalactosamine (8.7%, w/w), galactose (19.2%, w/w), mannose (4.1%, w/w), N-acetylneuraminic acid (13.8%, w/w) and sulphate (2.7%, w/w), was also obtained from preparations of reduced ovomucin by the density-gradient method. 4. Mild acid hydrolysis of the unfractionated ovomucin complex showed that N-acetylneuraminic acid occupied a terminal position of the oligosaccharide chains. 5. Alkaline beta-elimination reactions with the unfractionated ovomucin complex indicated that N-acetylgalactosamine was linked by alkali-labile bonds to hydroxy amino acids.
Topics: Amino Acids; Centrifugation, Density Gradient; Chromatography, Gel; Chromatography, Paper; Egg White; Electrophoresis; Galactosamine; Galactose; Glucosamine; Glycoproteins; Hexosamines; Hexoses; Hydrolysis; Mannose; Models, Structural; Molecular Weight; Neuraminic Acids; Oxidation-Reduction; Sulfates
PubMed: 5119791
DOI: 10.1042/bj1210537 -
The Journal of Biological Chemistry Oct 1978A chromatographic procedure for purification of the proteins in human parotid saliva has been developed. The eluates of a Sephadex G-150 and two ion exchange columns...
A chromatographic procedure for purification of the proteins in human parotid saliva has been developed. The eluates of a Sephadex G-150 and two ion exchange columns have been analyzed simultaneously by several physical and chemical tests; these include three optical properties of proteins, assays for neutral sugars, sialic acid and zinc, and disc gel electrophoresis. The ratios of the different variables have been used to determine the homogeneity and complexity of the protein distribution in the various peaks of the chromatographic eluates. By chromatographic methods, it has been possible to purify a glycoprotein with unusual staining characteristics and amino acid composition. Glycoproteins with similar properties comprise a major portion of the proteins in parotid saliva and appear to constitute a family of related proteins which differ in molecular size, carbohydrate and sialic acid content, and electrophoretic mobility. The fractionation of several enzymes in parotid saliva is also reported.
Topics: Amino Acids; Carbohydrates; Glycoproteins; Humans; Hydrolases; Molecular Weight; Parotid Gland; Proteins; Saliva; Sialic Acids; Spectrophotometry, Ultraviolet; Zinc
PubMed: 701270
DOI: No ID Found -
The Biochemical Journal Dec 1973A glycoprotein was isolated from the M-1 acid glycoprotein fraction of human colostrum. It had a molecular weight of 31200 and contained 27% galactose, 21.7% hexosamine,...
A glycoprotein was isolated from the M-1 acid glycoprotein fraction of human colostrum. It had a molecular weight of 31200 and contained 27% galactose, 21.7% hexosamine, 8.0% fucose and 10.8% sialic acid by weight. The glycoprotein had no absorption maxima in the 240-300nm region, and was virtually free of ABH(O) and M and N blood-group activity. Alkaline borohydride cleavage of the glycoprotein resulted predominantly in the destruction of threonine and galactosamine.
Topics: Borohydrides; Chromatography; Chromatography, DEAE-Cellulose; Colostrum; Electrophoresis; Female; Fucose; Galactosamine; Galactose; Glycoproteins; Hexosamines; Humans; Molecular Weight; Neuraminic Acids; Pregnancy; Spectrophotometry; Threonine; Ultracentrifugation
PubMed: 4778282
DOI: 10.1042/bj1350875 -
The Journal of Biological Chemistry Dec 1976Five gangliosides isolated from human kidney have been characterized. The two main fractions were shown to be typical extraneural gangliosides in having lactose as their...
Five gangliosides isolated from human kidney have been characterized. The two main fractions were shown to be typical extraneural gangliosides in having lactose as their neutral carbohydrate moiety. Their structures were identified as: AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-8)AcNeu(alpha2-3)Gal(beta1-4)Glc(beta1-1)Cer. The two main hexosamine-containing gangliosides are structurally related to human blood group substances of glycosphingolipid nature. The following structures are postulated: AcNeu(alpha2-3)Gal(beta1-4)GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1)Cer and AcNeu(alpha2-3)Gal(beta1-4)[Fuc(alpha1-3)]GlcNAc(beta1-3)Gal(beta1-4)Glc(beta1-1)Cer. The third hexosamine-containing ganglioside belongs to a different series of glycolipids and was shown to have the structure of a major ganglioside of human brain: AcNeu(alpha2-3)Gal(beta1-3)GalNAc(beta1-4)[AcNeu(alpha2-3)]Gal(beta1-4)Glc(beta1-1)Cer. The fatty acid structure of different gangliosides was shown to resemble that of neutral glycolipids of human kidney with the nonhydroxy acids C16:0, C22:0, and C24:0 as major components.
Topics: Acetylgalactosamine; Acetylglucosamine; Fatty Acids; Fucose; Galactose; Gangliosides; Glucose; Humans; Kidney; Mass Spectrometry; Molecular Conformation; Sialic Acids
PubMed: 1002698
DOI: No ID Found -
The Biochemical Journal Jul 1971Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved...
Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate composition. Wide variation was observed in the content of galactose, N-acetylglucosamine and sialic acid. Charge heterogeneity was related, in part, to variation in sialic acid content. The implications of variable carbohydrate composition for the structure and function of ovomucoid are discussed.
Topics: Amino Acids; Animals; Chickens; Chromatography; Chromatography, DEAE-Cellulose; Egg White; Galactose; Glucosamine; Glycoproteins; Hexoses; Immunochemistry; Immunodiffusion; Mannose; Neuraminic Acids; Trypsin Inhibitors
PubMed: 5001592
DOI: 10.1042/bj1230399 -
European Journal of Biochemistry Sep 1985The structure of the polysaccharide part of the lipopolysaccharide from Bacteroides fragilis NCTC 9343 has been determined using sugar and methylation analysis as the...
The structure of the polysaccharide part of the lipopolysaccharide from Bacteroides fragilis NCTC 9343 has been determined using sugar and methylation analysis as the principal tools. Phenol--water extraction followed by a phenol--chloroform--light petroleum extraction yielded a lipopolysaccharide suitable for structural analysis. Analysis of sugars using alditol acetates showed that the polysaccharide contained L-rhamnose, D-galactose and D-glucose in the approximate molar ratios of 1:5:1. After weak acid hydrolysis, two polysaccharide fractions were isolated by gel permeation chromatography: PSI and PSII with the sugar molar ratios 1:5:1 and 1:2:1 respectively. Chromium trioxide oxidation revealed that all galactosyl residues have the beta configuration, and that the rhamnosyl and glucosyl residues have the alpha configuration. From methylation analysis of lipopolysaccharide and the PS I and PS II fractions the following structures could be deduced.
Topics: Bacteroides fragilis; Carbohydrate Conformation; Cell Wall; Chemical Phenomena; Chemistry; Electrophoresis, Polyacrylamide Gel; Fatty Acids; Gas Chromatography-Mass Spectrometry; Hydrolysis; Lipopolysaccharides; Methylation; Polysaccharides
PubMed: 4029152
DOI: 10.1111/j.1432-1033.1985.tb09154.x -
European Journal of Biochemistry Sep 1983The fraction GP-IV obtained from hen ovalbumin has been further fractionated on DEAE-Sephadex A-25 in borate buffer, a new system for the separation and isolation of...
The fraction GP-IV obtained from hen ovalbumin has been further fractionated on DEAE-Sephadex A-25 in borate buffer, a new system for the separation and isolation of glycoasparagines on columns. The structures of one major and two minor glycoasparagines thus obtained were determined by enzymatic (endo-beta-N-acetylglucosaminidase D and H digestion), chemical (modified Smith degradation, acetolysis, and methylation analysis), and instrumental (270 MHz 1H nuclear magnetic resonance spectroscopy) analyses. The following structure is novel for carbohydrate moieties in ovalbumin glycoasparagines: (formula; see text) The present anion exchange chromatography in borate solution is well suited for separating and preparing oligosaccharides having similar molecular size but differing in content of vicinal cis-diol group.
Topics: Animals; Borates; Chemical Phenomena; Chemistry; Chickens; Chromatography, Ion Exchange; Egg White; Glycopeptides; Ovalbumin
PubMed: 6884363
DOI: 10.1111/j.1432-1033.1983.tb07644.x -
European Journal of Biochemistry Jun 1981In the nervous tissue of miniature pig, type Göttingen, three fucosyl-containing gangliosides are major gangliosides. All three were found in dorsal-root ganglia and...
In the nervous tissue of miniature pig, type Göttingen, three fucosyl-containing gangliosides are major gangliosides. All three were found in dorsal-root ganglia and spinal cord, but only two of them in the forebrain. The concentrations of these gangliosides were increased in chloroquine intoxication. Their chemical structures were determined by component analysis, partial acid and enzymatic degradation and assay of the partially methylated sugars after hydrolysis, reduction and acetylation. Mass spectrometric analysis of the intact gangliosides as permethylated, permethylated-reduced and permethylated-reduced-silylated derivatives confirmed the following structures of the three gangliosides. (See Formula in text).
Topics: Animals; Brain Chemistry; Carbohydrate Conformation; Carbohydrate Sequence; Fucose; Ganglia, Spinal; Gangliosides; Mass Spectrometry; Sialic Acids; Sphingosine; Spinal Cord; Swine
PubMed: 7262075
DOI: 10.1111/j.1432-1033.1981.tb05372.x -
Journal of Bacteriology Jun 1973Phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. Results showed that phospholipids of marine bacteria differed very little from...
Phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. Results showed that phospholipids of marine bacteria differed very little from those of nonmarine organisms with phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol being the predominant phospholipids in all strains examined. Lyso-phosphatidylethanolamine occurred in significant quantities among a number of the marine bacteria, and two of the isolates contained significant quantities of poly-beta-hydroxybutyrate. Effects of age and growth temperature on the phospholipid composition were also investigated. It is suggested that phylogenetic relationships among bacteria may be correlated with phospholipid composition.
Topics: Age Factors; Alcaligenes; Arthrobacter; Bacteria; Chromatography, Thin Layer; Culture Media; Hydroxybutyrates; Lipids; Phosphates; Phosphatidylethanolamines; Phospholipids; Photobacterium; Pseudomonas aeruginosa; Spirillum; Temperature; Vibrio
PubMed: 4197274
DOI: 10.1128/jb.114.3.897-908.1973