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The Biochemical Journal Jan 19721. Qualitative and quantitative analytical results for the lipopolysaccharide from acetone-dried cells of Pseudomonas aeruginosa (N.C.T.C. 1999) are presented and...
1. Qualitative and quantitative analytical results for the lipopolysaccharide from acetone-dried cells of Pseudomonas aeruginosa (N.C.T.C. 1999) are presented and possible contamination of the material with nucleic acid was further examined. 2. Additional sugars detected (only in large-scale hydrolysates) were mannose and arabinose; traces of spermidine and putrescine were also found. 3. The heptose component is l-glycero-d-mannoheptose. 4. The thiobarbituric acid-positive component is a 3-deoxy-2-octulonic acid, of which only 35-40% links lipid A to the polysaccharide. This linkage is not broken by hydrolysis with acetic acid up to 0.08m. 5. Liberation of lipid A required hydrolysis with 0.1m-hydrochloric acid, which substantially degraded the polysaccharide moiety. 6. Fractions obtained from the degraded polysaccharide by high-voltage electrophoresis were examined; in these, the alanine/galactosamine molar ratio is approx. 1. 7. Hydrazinolysis of whole lipopolysaccharide showed that at least 40% of the alanine is in amide linkage, possibly with galactosamine. 8. Lipid A, solubilized by alkaline methanolysis was fractionated; most of the phosphorus of the higher-molecular-weight fractions was released as P(i) by a phosphomonoesterase. 9. Hydrazinolysis of lipid A destroyed approx. 80% of the glucosamine, and glycosidically linked glucosamine oligosaccharides could not be isolated.
Topics: Alanine; Arabinose; Chemical Phenomena; Chemistry; Chromatography, Gas; Chromatography, Gel; Chromatography, Paper; Electrophoresis, Paper; Fucose; Galactosamine; Glucosamine; Heptoses; Hexosamines; Hydrazines; Hydrolysis; Lipids; Lipopolysaccharides; Mannose; Models, Chemical; Phosphoric Monoester Hydrolases; Polysaccharides, Bacterial; Pseudomonas aeruginosa; Putrescine; Spermidine
PubMed: 4627191
DOI: 10.1042/bj1260395 -
The Biochemical Journal Jul 19711. Three peptides containing alpha-aminoadipic acid and cysteine have been obtained in small amounts from the mycelium of a Cephalosporium sp. 2. The peptides were...
1. Three peptides containing alpha-aminoadipic acid and cysteine have been obtained in small amounts from the mycelium of a Cephalosporium sp. 2. The peptides were precipitated as cuprous mercaptides together with glutathione and resolved from the latter and from each other by preparative paper electrophoresis and chromatography either in the sulphonic acid form or as S-sulphonyl derivatives. From the S-sulphonyl derivatives they were obtained in the thiol form. 3. One peptide (P3) was shown by amino acid analysis and the mass spectrum of the NS-ethoxycarbonyl derivative of its methyl ester to be delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine. A second peptide (P2) contained alpha-aminoadipic acid, cysteine, valine and glycine, and the third peptide (P1) contained alpha-aminoadipic acid, cysteine, beta-hydroxyvaline and glycine.
Topics: Acremonium; Adipates; Chromatography; Copper; Cysteine; Electrophoresis; Esters; Glutathione; Glycine; Mass Spectrometry; Peptides; Sulfhydryl Compounds; Sulfonic Acids; Valine
PubMed: 5166548
DOI: 10.1042/bj1230471 -
Journal of Bacteriology Jun 1973Phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. Results showed that phospholipids of marine bacteria differed very little from...
Phospholipid compositions of 20 strains of marine and estuarine bacteria were determined. Results showed that phospholipids of marine bacteria differed very little from those of nonmarine organisms with phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol being the predominant phospholipids in all strains examined. Lyso-phosphatidylethanolamine occurred in significant quantities among a number of the marine bacteria, and two of the isolates contained significant quantities of poly-beta-hydroxybutyrate. Effects of age and growth temperature on the phospholipid composition were also investigated. It is suggested that phylogenetic relationships among bacteria may be correlated with phospholipid composition.
Topics: Age Factors; Alcaligenes; Arthrobacter; Bacteria; Chromatography, Thin Layer; Culture Media; Hydroxybutyrates; Lipids; Phosphates; Phosphatidylethanolamines; Phospholipids; Photobacterium; Pseudomonas aeruginosa; Spirillum; Temperature; Vibrio
PubMed: 4197274
DOI: 10.1128/jb.114.3.897-908.1973 -
European Journal of Biochemistry Jun 1981In the nervous tissue of miniature pig, type Göttingen, three fucosyl-containing gangliosides are major gangliosides. All three were found in dorsal-root ganglia and...
In the nervous tissue of miniature pig, type Göttingen, three fucosyl-containing gangliosides are major gangliosides. All three were found in dorsal-root ganglia and spinal cord, but only two of them in the forebrain. The concentrations of these gangliosides were increased in chloroquine intoxication. Their chemical structures were determined by component analysis, partial acid and enzymatic degradation and assay of the partially methylated sugars after hydrolysis, reduction and acetylation. Mass spectrometric analysis of the intact gangliosides as permethylated, permethylated-reduced and permethylated-reduced-silylated derivatives confirmed the following structures of the three gangliosides. (See Formula in text).
Topics: Animals; Brain Chemistry; Carbohydrate Conformation; Carbohydrate Sequence; Fucose; Ganglia, Spinal; Gangliosides; Mass Spectrometry; Sialic Acids; Sphingosine; Spinal Cord; Swine
PubMed: 7262075
DOI: 10.1111/j.1432-1033.1981.tb05372.x -
The Biochemical Journal Jul 1971Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved...
Three major and two minor species of ovomucoid were separated by chromatography on sulphoethyl-Sephadex. The predominant sialic acid-free species was further resolved into three fractions by DEAE-cellulose chromatography. Although all species of ovomucoid had closely similar trypsin-inhibiting activity, immunochemical properties and amino acid composition, they differ in carbohydrate composition. Wide variation was observed in the content of galactose, N-acetylglucosamine and sialic acid. Charge heterogeneity was related, in part, to variation in sialic acid content. The implications of variable carbohydrate composition for the structure and function of ovomucoid are discussed.
Topics: Amino Acids; Animals; Chickens; Chromatography; Chromatography, DEAE-Cellulose; Egg White; Galactose; Glucosamine; Glycoproteins; Hexoses; Immunochemistry; Immunodiffusion; Mannose; Neuraminic Acids; Trypsin Inhibitors
PubMed: 5001592
DOI: 10.1042/bj1230399 -
The Journal of Investigative Dermatology Jun 1978
Topics: Animals; Ceramides; Chemical Fractionation; Chromatography, Thin Layer; Fatty Acids; Glycosphingolipids; Humans; Skin; Swine
PubMed: 649981
DOI: 10.1111/1523-1747.ep12543527 -
Journal of Bacteriology Feb 1976A new heteropolysaccharide has been isolated by alkaline extraction of hyphal walls of Aspergillus niger NRRL 326 grown in surface culture. Its composition by weight, as...
A new heteropolysaccharide has been isolated by alkaline extraction of hyphal walls of Aspergillus niger NRRL 326 grown in surface culture. Its composition by weight, as determined by paper and gas chromatography and colorimetric analyses, is 70% galactose, 20% galactosamine, 6% glucose, and 1% acetyl. Two independent experiments have been used to ascertain copolymer structure: permeation chromatography in 6 M guanidinium hydrochloride, with controlled-pore glass columns of two fractionation ranges, and nitrous acid deaminative cleavage of galactosaminogalactan followed by reduction of fragments with [3H]borohydride and gel filtration chromatography. One of the tritiated fragments is tentatively identified as the disaccharide derivative galactopyranosyl 2,5-anhydrotalitol, on the basis of chromatographic properties and by kinetics of its acid hydrolysis. Smith degradation, methylation, deamination, and optical rotation studies indicate that the galactosaminogalactan consists of a linear array of hexopyranosyl units joined almost exclusively by alpha-(1 leads to 4) linkages. Hexosaminyl moieties are distributed randomly along the chains, which have an average degree of polymerization of about 100. The possible significance of this macromolecule in hyphal structure is considered.
Topics: Aspergillus; Aspergillus niger; Cell Wall; Chemical Precipitation; Chromatography; Galactosamine; Galactose; Glucose; Glycosides; Hydrolysis; Methylation; Oxidation-Reduction; Periodic Acid; Polysaccharides
PubMed: 173713
DOI: 10.1128/jb.125.2.655-669.1976 -
The Journal of Biological Chemistry May 1970
Comparative Study
Topics: Amino Acids; Chemical Phenomena; Chemistry; Chromatography, DEAE-Cellulose; Chromatography, Gel; Coenzymes; Electrophoresis; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Methods; Molecular Weight; Oxidoreductases; Peptides; Proteins; Saccharomyces; Spectrophotometry; Streptomycin; Sulfides; Ultracentrifugation; Ultraviolet Rays
PubMed: 5442277
DOI: No ID Found -
Journal of Biochemistry Apr 1984Neutral and acidic glycosphingolipids were purified from porcine pancreas by chromatography on columns of DEAE-Sephadex and Iatrobeads. The chemical structures of the...
Neutral and acidic glycosphingolipids were purified from porcine pancreas by chromatography on columns of DEAE-Sephadex and Iatrobeads. The chemical structures of the purified glycolipids were determined by carbohydrate analysis, methylation analysis, enzyme treatment, fatty acid analysis, NMR and IR. The major glycolipid of porcine pancreas was Gal(alpha,1-4)Gal(beta,1-)ceramide. Gangliosides GM3 and GD3 were major acidic components and galactosylceramide 3-sulfate was also found.
Topics: Animals; Carbohydrate Conformation; Carbohydrate Sequence; Carbohydrates; Chromatography, Gel; Chromatography, Ion Exchange; Fatty Acids; Glycoside Hydrolases; Glycosphingolipids; Magnetic Resonance Spectroscopy; Methylation; Pancreas; Spectrophotometry, Infrared; Swine
PubMed: 6746593
DOI: 10.1093/oxfordjournals.jbchem.a134702 -
The Journal of Biological Chemistry Aug 1978
Topics: Amino Acids; Carbohydrates; Cell Line; Glycoproteins; Molecular Conformation; Oligosaccharides; Sialic Acids; Vesicular stomatitis Indiana virus; Viral Proteins
PubMed: 209045
DOI: No ID Found